公开(公告)号：EP3186368A4

公开(公告)日：2018-04-11

申请号：EP15836904

申请日：2015-08-25

申请人： GENEWEAVE BIOSCIENCES INC

发明人： REY DIEGO ARIEL ,  REHFUSS MARC ,  LIU XIAOWEN

IPC分类号： C12N7/00 ,  C12N7/01 ,  C12N15/74

CPC分类号： C12N15/70 ,  C07K14/005 ,  C12N7/00 ,  C12N9/0069 ,  C12N9/16 ,  C12N15/74 ,  C12N2795/10122 ,  C12N2795/10143 ,  C12N2795/10152 ,  C12Q1/6897 ,  C12Y113/12007

摘要： Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.

公开(公告)号：EP1180153B1

公开(公告)日：2005-04-13

申请号：EP00932819.6

申请日：2000-05-24

申请人： Phage Biotechnology Corporation

发明人： KORDYUM, Vitaliy, A. ,  CHERNYKH, Svetlana, I. ,  SLAVCHENKO, Irina, Y. ,  VOZIANOV, Oleksandr, F.

IPC分类号： C12N15/73 ,  C07K14/60 ,  C12P21/02 ,  C12N7/00 ,  C12N1/06

CPC分类号： C12N7/00 ,  C07K14/60 ,  C12N1/06 ,  C12N15/73 ,  C12N2795/10143 ,  C12P21/02

摘要： This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.

公开(公告)号：EP3307872A1

公开(公告)日：2018-04-18

申请号：EP16812275.2

申请日：2016-06-15

申请人： North Carolina State University

发明人： BEISEL, Chase Lawrence ,  GOMAA, Ahmed Abdelshafy Mahmoud ,  LUO, Michelle

IPC分类号： C12N1/21 ,  C12N7/01 ,  C12N15/63 ,  C12P19/34

CPC分类号： C12N15/70 ,  A01N63/00 ,  A61K38/00 ,  A61P31/04 ,  C12N7/00 ,  C12N9/1007 ,  C12N9/22 ,  C12N9/96 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/20 ,  C12N2795/10143 ,  C12N2795/14143 ,  C12P19/34 ,  Y02A50/481

摘要： The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.

公开(公告)号：EP3108014A1

公开(公告)日：2016-12-28

申请号：EP15719021.6

申请日：2015-02-18

申请人： Laboratory Corporation of America Holdings

发明人： ANDERSON, Dwight Lyman ,  GIL, Jose S. ,  HOPKINS, Ben Barrett ,  ERICKSON, Stephen Eric

IPC分类号： C12Q1/70

CPC分类号： C12Q1/70 ,  C12N15/86 ,  C12N2795/10121 ,  C12N2795/10131 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10231 ,  C12N2795/10243

摘要： Disclosed herein are methods and systems for rapid detection of microorganisms in a sample, without culturing for enrichment of the microorganism. A modified bacteriophage is also disclosed which comprises a non-native indicator gene in the late gene region. The indicator product is not a fusion protein. The specificity of infectious agents allows a specific microorganism to be targeted, and an indicator signal may be amplified to optimize assay sensitivity.

摘要翻译： 公开用于快速检测样品中微生物的方法和系统，无需培养微生物的富集。 一种改进的噬菌体gibt游离缺失盘，它包括在晚期基因区中的非天然指示基因。 指示器产品不是融合蛋白。 传染性病原体的特异性允许一个特定的微生物待靶向，以及指示信​​号可以被放大，以优化测定法的灵敏度。

公开(公告)号：EP2761014A2

公开(公告)日：2014-08-06

申请号：EP12835856.1

申请日：2012-09-26

申请人： Sample6 Technologies Inc.

发明人： LU, Timothy, Kuan Ta ,  KOERIS, Michael, Sandor ,  CHEVALIER, Brett, Smith ,  HOLDER, Jason, Wyatt ,  MCKENZIE, Gregory, John ,  BROWNELL, Daniel, Robert

IPC分类号： C12P19/34

CPC分类号： C12N7/00 ,  C12N15/10 ,  C12N15/81 ,  C12N15/86 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10243 ,  C12N2795/10251 ,  C12N2800/206 ,  C12P19/34

摘要： This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

公开(公告)号：EP1180153A1

公开(公告)日：2002-02-20

申请号：EP00932819.6

申请日：2000-05-24

申请人： Phage Biotechnology Corporation

发明人： KORDYUM, Vitaliy, A. ,  CHERNYKH, Svetlana, I. ,  SLAVCHENKO, Irina, Y. ,  VOZIANOV, Oleksandr, F.

IPC分类号： C12N15/73 ,  C07K14/60 ,  C12P21/02 ,  C12N7/00 ,  C12N1/06

CPC分类号： C12N7/00 ,  C07K14/60 ,  C12N1/06 ,  C12N15/73 ,  C12N2795/10143 ,  C12P21/02

摘要： This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.

公开(公告)号：EP3108014B1

公开(公告)日：2018-12-05

申请号：EP15719021.6

申请日：2015-02-18

申请人： Laboratory Corporation of America Holdings

发明人： ANDERSON, Dwight Lyman ,  GIL, Jose S. ,  HOPKINS, Ben Barrett ,  ERICKSON, Stephen Eric

IPC分类号： C12Q1/70

CPC分类号： C12Q1/70 ,  C12N15/86 ,  C12N2795/10121 ,  C12N2795/10131 ,  C12N2795/10143 ,  C12N2795/10221 ,  C12N2795/10231 ,  C12N2795/10243

摘要： Disclosed herein are methods and systems for rapid detection of microorganisms in a sample, without culturing for enrichment of the microorganism. A modified bacteriophage is also disclosed which comprises a non-native indicator gene in the late gene region. The indicator product is not a fusion protein. The specificity of infectious agents allows a specific microorganism to be targeted, and an indicator signal may be amplified to optimize assay sensitivity.

公开(公告)号：EP3186368A1

公开(公告)日：2017-07-05

申请号：EP15836904.1

申请日：2015-08-25

申请人： Geneweave Biosciences Inc.

发明人： REY, Diego, Ariel ,  REHFUSS, Marc ,  LIU, Xiaowen

IPC分类号： C12N7/00 ,  C12N7/01 ,  C12N15/74

CPC分类号： C12N15/70 ,  C07K14/005 ,  C12N7/00 ,  C12N9/0069 ,  C12N9/16 ,  C12N15/74 ,  C12N2795/10122 ,  C12N2795/10143 ,  C12N2795/10152 ,  C12Q1/6897 ,  C12Y113/12007

摘要： Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.

摘要翻译： 提供了用于将报告核酸分子包装到非复制转导颗粒中用作报告分子的方法和系统。 非复制型转导颗粒可以由病毒构建并使用病毒转导和复制系统。 报道核酸分子包括报道基因，例如报道分子或选择标记，用于检测靶基因或细胞。 提供了使用非复制转导颗粒作为报道分子检测细胞和靶核酸分子的方法和系统。

公开(公告)号：EP2171069A4

公开(公告)日：2011-01-05

申请号：EP08775536

申请日：2008-07-04

申请人： FINNZYMES OY

发明人： SAVILAHTI HARRI

IPC分类号： C12N15/85 ,  C12N5/22 ,  C12N15/90

CPC分类号： C12N15/86 ,  C12N15/907 ,  C12N2795/10143 ,  C12N2800/90

公开(公告)号：EP2171069A1

公开(公告)日：2010-04-07

申请号：EP08775536.9

申请日：2008-07-04

申请人： Finnzymes Oy

发明人： SAVILAHTI, Harri

IPC分类号： C12N15/85 ,  C12N5/22

CPC分类号： C12N15/86 ,  C12N15/907 ,  C12N2795/10143 ,  C12N2800/90

摘要： The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for isolated human stem cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of isolated human stem cells. The kit can be used, e.g., to generate insertional mutant libraries.