公开(公告)号：EP3283629A1

公开(公告)日：2018-02-21

申请号：EP16780825.2

申请日：2016-04-15

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  ZHANG, Huidan ,  HEYMAN, John ,  KLEIN, Allon, Moshe

IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2523/319 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/179

摘要： The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.

公开(公告)号：EP3039162B1

公开(公告)日：2019-01-02

申请号：EP14840425.4

申请日：2014-08-22

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  HEYMAN, John ,  GILBERT, John, R.

IPC分类号： C12P21/04 ,  C12Q1/6881 ,  C12Q1/02 ,  C40B30/04 ,  C40B30/06 ,  G01N33/00 ,  G01N33/50 ,  G01N33/566

公开(公告)号：EP3039162A1

公开(公告)日：2016-07-06

申请号：EP14840425.4

申请日：2014-08-22

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  HEYMAN, John ,  GILBERT, John, R.

IPC分类号： C12Q1/68 ,  G01N33/00 ,  C12P21/04 ,  C40B30/04 ,  C40B30/06

CPC分类号： C12Q1/6881 ,  C12Q1/02 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/70517

摘要： The present invention generally relates to fluidic droplets, and to techniques for screening or sorting such fluidic droplets. In some embodiments, the fluidic droplets may contain cells such as immune cells, which can be analyzed to determine receptor sequences or other useful properties of the cells. For example, in one aspect, the present invention is generally related to determining immune cell receptors by encapsulating immune cells and target cells in microfluidic droplets, determining the effect of the immune cells on the target cells, and for those immune cells that kill or otherwise adversely affect the target cells, determining one or more receptor sequences of those immune cells. The target cells may be, for example, cancer cells or virally-infected cells. In some cases, the receptor sequences can be used, for example, to identify certain properties of the immune cells, to screen for drugs or other therapeutic agents, or the like.

摘要翻译： 本发明一般涉及流体液滴，以及用于筛选或分类这种流体液滴的技术。 在一些实施方案中，流体液滴可以含有诸如免疫细胞的细胞，其可被分析以确定受体序列或细胞的其它有用特性。 例如，在一个方面，本发明通常涉及通过将免疫细胞和靶细胞包封在微流体液滴中来确定免疫细胞受体，确定免疫细胞对靶细胞的影响以及杀死或以其他方式的那些免疫细胞的作用 不利地影响靶细胞，确定那些免疫细胞的一个或多个受体序列。 靶细胞可以是例如癌细胞或病毒感染的细胞。 在一些情况下，受体序列可以用于例如鉴定免疫细胞的某些特性，筛选药物或其它治疗剂等。