公开(公告)号：EP4090740A1

公开(公告)日：2022-11-23

申请号：EP21741543.9

申请日：2021-01-13

Applicant: President and Fellows of Harvard College

Inventor： WEITZ, David, A. ,  ZHANG, Huidan

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6834

公开(公告)号：EP3481771A1

公开(公告)日：2019-05-15

申请号：EP17824956.1

申请日：2017-07-07

Applicant: President and Fellows of Harvard College

Inventor： WEITZ, David, A. ,  ZHANG, Huidan ,  CUI, Nai, Wen

IPC: C01C1/14 ,  C01C1/24 ,  C12N15/10 ,  G01N33/50

公开(公告)号：EP3283629A1

公开(公告)日：2018-02-21

申请号：EP16780825.2

申请日：2016-04-15

Applicant: President and Fellows of Harvard College

Inventor： WEITZ, David, A. ,  ZHANG, Huidan ,  HEYMAN, John ,  KLEIN, Allon, Moshe

IPC: C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12P19/34 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2523/319 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.

公开(公告)号：EP3481540A1

公开(公告)日：2019-05-15

申请号：EP17824957.9

申请日：2017-07-07

Applicant: President and Fellows of Harvard College ,  Massachusetts Eye & Ear Infirmary

Inventor： WEITZ, David, A. ,  ZHANG, Huidan ,  CUI, Nai, Wen ,  LEI, Fengyang ,  ILIOS, Eleftherios, Paschalis

IPC: B01J2/08 ,  B01J13/02 ,  B01J19/00

公开(公告)号：EP3268125A1

公开(公告)日：2018-01-17

申请号：EP16765491.2

申请日：2016-03-11

Applicant: President and Fellows of Harvard College

Inventor： WEITZ, David, A. ,  ZHANG, Huidan

IPC: B01J19/00 ,  B32B5/16 ,  C12N9/00 ,  C12N11/00 ,  C12P21/04 ,  C40B30/04 ,  C40B40/10

CPC classification number: C12Q1/686 ,  C12N15/1075 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2531/113

Abstract: The present invention generally relates to microfluidics and, in particular, to systems and methods for determining cells using amplification. In one set of embodiments, cells are encapsulated within droplets and nucleic acids from the cells amplified within the droplets. The droplets may then be pooled together and the amplified nucleic acids can be determined using PCR or other suitable techniques. In some embodiments, techniques such as these can be used to detect relatively rare cells that may be present, e.g., if the droplets are amplified using conditions able to selectively amplify nucleic acids arising from the relatively rare cells.