公开(公告)号：EP2931305A1

公开(公告)日：2015-10-21

申请号：EP13862430.9

申请日：2013-12-12

申请人： Promega Corporation

发明人： HITKO, Carolyn W. ,  HURST, Robin ,  KIRKLAND, Thomas ,  KLAUBERT, Dieter ,  MEISENHEIMER, Poncho ,  NATH, Nidhi ,  OHANA, Rachel F. ,  OTTO, Paul ,  URH, Marjeta ,  UYEDA, Harry Tetsuo ,  WOOD, Keith

IPC分类号： A61K39/00

CPC分类号： G01N33/54333 ,  G01N33/54306 ,  G01N33/54326

摘要： The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

公开(公告)号：EP2931305B1

公开(公告)日：2018-11-28

申请号：EP13862430.9

申请日：2013-12-12

申请人： Promega Corporation

发明人： HITKO, Carolyn W. ,  HURST, Robin ,  KIRKLAND, Thomas ,  KLAUBERT, Dieter ,  MEISENHEIMER, Poncho ,  NATH, Nidhi ,  OHANA, Rachel F. ,  OTTO, Paul ,  URH, Marjeta ,  UYEDA, Harry Tetsuo ,  WOOD, Keith

IPC分类号： A61K39/00 ,  G01N33/543

CPC分类号： G01N33/54333 ,  G01N33/54306 ,  G01N33/54326

摘要： The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

公开(公告)号：EP2197996B1

公开(公告)日：2012-12-05

申请号：EP08838555.4

申请日：2008-10-10

申请人： Promega Corporation

发明人： SAVELIEV, Sergei ,  SIMPSON, Daniel ,  WOOD, Keith V. ,  UYEDA, Harry Tetsuo ,  WOODROOFE HITKO, Carolyn ,  KLAUBERT, Dieter

IPC分类号： C11D1/12 ,  G01N33/68

CPC分类号： C11D1/20 ,  C07C309/10 ,  C07C309/14 ,  C07C309/15 ,  C07C309/16 ,  C07C323/12 ,  C07C329/06 ,  C07D307/40 ,  C07D307/56 ,  C07D317/22 ,  C07D333/16 ,  C11D1/143

摘要： The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilisation, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

公开(公告)号：EP4010327A1

公开(公告)日：2022-06-15

申请号：EP20761015.5

申请日：2020-08-06

申请人： Promega Corporation

发明人： WALKER, Joel ,  SAVELIEV, Sergei ,  ZENG, Zhiyang ,  KARGBO, Robert ,  WANG, Hui ,  LEVIN, Sergiy ,  RESSLER, Valerie ,  ZHANG, Jun ,  SHI, Ce ,  UYEDA, Harry Tetsuo ,  OSTERMAN, Jean ,  ZHOU, Min ,  RAI, Prabin ,  ZHOU, Wenhui

IPC分类号： C07D317/24 ,  C07C309/15 ,  C12P21/06

公开(公告)号：EP2969435A1

公开(公告)日：2016-01-20

申请号：EP14769196.8

申请日：2014-03-13

申请人： Promega Corporation

发明人： HITKO, Carolyn W. ,  KIRKLAND, Thomas ,  LEVIN, Sergiy ,  MEISENHEIMER, Poncho ,  OHANA, Rachel Friedman ,  UYEDA, Harry Tetsuo ,  ZHU, Ji

IPC分类号： B27L11/00

CPC分类号： C12Q1/34 ,  C12N9/14 ,  G01N33/54353

摘要： The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.