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公开(公告)号:EP2197996B1
公开(公告)日:2012-12-05
申请号:EP08838555.4
申请日:2008-10-10
申请人: Promega Corporation
发明人: SAVELIEV, Sergei , SIMPSON, Daniel , WOOD, Keith V. , UYEDA, Harry Tetsuo , WOODROOFE HITKO, Carolyn , KLAUBERT, Dieter
CPC分类号: C11D1/20 , C07C309/10 , C07C309/14 , C07C309/15 , C07C309/16 , C07C323/12 , C07C329/06 , C07D307/40 , C07D307/56 , C07D317/22 , C07D333/16 , C11D1/143
摘要: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilisation, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.
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公开(公告)号:EP2614059B1
公开(公告)日:2015-11-18
申请号:EP11763816.3
申请日:2011-09-09
申请人: Promega Corporation
IPC分类号: C07D417/04 , C07K5/10 , C07K7/00 , G01N33/68
CPC分类号: C12Q1/34 , C07D417/04 , C07K5/0806 , C07K5/1019 , C07K7/06 , C12Q1/48
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公开(公告)号:EP2614059A2
公开(公告)日:2013-07-17
申请号:EP11763816.3
申请日:2011-09-09
申请人: Promega Corporation
IPC分类号: C07D417/04 , C07K5/10 , C07K7/00 , G01N33/68
CPC分类号: C12Q1/34 , C07D417/04 , C07K5/0806 , C07K5/1019 , C07K7/06 , C12Q1/48
摘要: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.
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