公开(公告)号：EP2220105A2

公开(公告)日：2010-08-25

申请号：EP08738278.4

申请日：2008-04-30

申请人： Protalix Ltd.

发明人： SHAALTIEL, Yoseph ,  BAUM, Gideon ,  BARTFELD, Daniel ,  HASHMUELI, Sharon ,  LEWKOWICZ, Ayala

IPC分类号： C07H21/04 ,  C07K14/00

CPC分类号： A61K38/47 ,  A61K9/0053 ,  A61K36/23 ,  C07K2319/04 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/2465 ,  C12N15/8257 ,  C12P21/005 ,  C12Y302/01022 ,  C12Y302/01045

摘要： A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.

摘要翻译： 用于在植物培养物中产生糖基化蛋白质的装置，系统和方法，特别是具有高甘露糖糖基化的蛋白质，同时用ER信号靶向这种蛋白质和/或旁路高尔基体。 本发明还涉及使用转基因植物根特别是胡萝卜细胞表达和产生酶促活性的高甘露糖溶酶体酶的载体和方法。 更具体地，本发明涉及宿主细胞，特别是转基因悬浮胡萝卜细胞，用于高产量表达和生产生物活性高甘露糖葡糖脑苷脂酶（GCD）的载体和方法。 本发明还提供了用于治疗溶酶体贮积病的组合物和方法。

公开(公告)号：EP1618177B1

公开(公告)日：2012-06-06

申请号：EP04713966.2

申请日：2004-02-24

申请人： Protalix Ltd.

发明人： SHAALTIEL, Yoseph ,  BAUM, Gideon ,  BARTFELD, Daniel ,  HASHMUELI, Sharon ,  LEWKOWICZ, Ayala

IPC分类号： C12N15/82 ,  C12N9/24

CPC分类号： C12P21/005 ,  C07K2319/04 ,  C12N9/2402 ,  C12N15/8257 ,  C12Y302/01045

摘要： A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.

公开(公告)号：EP1618177A2

公开(公告)日：2006-01-25

申请号：EP04713966.2

申请日：2004-02-24

申请人： Protalix Ltd.

发明人： SHAALTIEL, Yoseph ,  BAUM, Gideon ,  BARTFELD, Daniel ,  HASHMUELI, Sharon ,  LEWKOWICZ, Ayala

IPC分类号： C12N1/00

CPC分类号： C12P21/005 ,  C07K2319/04 ,  C12N9/2402 ,  C12N15/8257 ,  C12Y302/01045

摘要： A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.