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1.发明公开VARIANTS DE BETA-GLUCOSIDASE A ACTIVITE AMELIOREE ET LEURS UTILISATIONS 有权具有改善的活性和β-葡萄糖苷酶OPTIONS及其用途
公开(公告)号：EP2342329A1
公开(公告)日：2011-07-13
申请号：EP09741395.9
申请日：2009-09-10
申请人： IFP Energies nouvelles , Proteus
CPC分类号： C12P7/04 , C12N9/2445 , C12P7/10 , C12P7/16 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more specifically to beta-glucosidase variants comprising at least one deterioration among the amino acids located at positions 225, 238, 240, and 241 according to the numbering in SEQ ID NO:2 of beta-glucosidase for Trichoderma reesei, and also relates to the use of said variants, having improved performance, in methods for cellulose decomposition and biofuel production.
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公开(公告)号：EP1404830B1
公开(公告)日：2008-07-16
申请号：EP02743542.9
申请日：2002-05-16
申请人： Proteus
IPC分类号： C12N15/10
CPC分类号： C12N15/1027
摘要： Method of preparing fragments for shuffling using DNA repair enzymes. Repair enzymes can be used to repair damaged and mismatched residues to reduce level of mutation in fragments, or can themselves create fragments when used without sufficient DNA polymerase. Invention further includes reaction mixtures created by or during the method, sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
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3.发明授权VARIANTS DE BETA-GLUCOSIDASE A ACTIVITE AMELIOREE ET LEURS UTILISATIONS 有权具有改善的活性和β-葡萄糖苷酶OPTIONS及其用途
公开(公告)号：EP2342329B1
公开(公告)日：2016-11-09
申请号：EP09741395.9
申请日：2009-09-10
申请人： IFP Énergies nouvelles , Proteus
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4.发明公开METHODS OF GENERATING MODIFIED POLYNUCLEOTIDE LIBRARIES AND METHODS OF USING THE SAME FOR DIRECTED PROTEIN EVOLUTION 审中-公开制造用于使用修饰的多核苷酸图书馆和方法为寻找蛋白开发
公开(公告)号：EP2526192A2
公开(公告)日：2012-11-28
申请号：EP11705258.9
申请日：2011-01-21
申请人： Proteus
IPC分类号： C12N15/10
CPC分类号： C12N15/1058 , C12N15/1027 , C12N15/1093 , C12N15/66
摘要： The invention provides for methods of generating modified polynucleotide libraries by inserting and/or deleting at least three nucleotide residues in polynucleotide sequences. Theses methods may be used with other methods of gene modification such as gene shuffling. The invention further provides methods of directed molecular evolution using the modified polynucleotide libraries produced by these methods.
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5.发明授权
公开(公告)号：EP1774032B1
公开(公告)日：2010-04-21
申请号：EP05775328.7
申请日：2005-06-07
申请人： Proteus
CPC分类号： C12N15/102 , G06F19/22
摘要： The invention relates to a method of determining the mutational load of a gene library obtained by random mutagenesis of a particular gene of interest and means for implementing same, comprising the following steps: a) preparation of an abacus that links the mutational load (CM) of a gene library obtained by random mutagenesis of a model gene to the fraction of mutated model genes observed in said library; b) random mutagenesis of the model gene that was used to prepare the abacus in step (a) and of the gene of interest, in order to produce the corresponding mutated gene libraries; c) determination of the mutational load (CM) of the gene library obtained from the model gene in step (b) using the abacus established in step (a); and d) application of a correction factor (CF) to the mutational load (CM) of the mutated model gene library determined in step (C), in order to determine the mutational load (CM) of the library of mutated genes of interest from step (b).
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6.发明公开
公开(公告)号：EP1774032A2
公开(公告)日：2007-04-18
申请号：EP05775328.7
申请日：2005-06-07
申请人： Proteus
CPC分类号： C12N15/102 , G06F19/22
摘要： The invention relates to a method of determining the mutational load of a gene library obtained by random mutagenesis of a particular gene of interest and means for implementing same, comprising the following steps: a) preparation of an abacus that links the mutational load (CM) of a gene library obtained by random mutagenesis of a model gene to the fraction of mutated model genes observed in said library; b) random mutagenesis of the model gene that was used to prepare the abacus in step (a) and of the gene of interest, in order to produce the corresponding mutated gene libraries; c) determination of the mutational load (CM) of the gene library obtained from the model gene in step (b) using the abacus established in step (a); and d) application of a correction factor (CF) to the mutational load (CM) of the mutated model gene library determined in step (C), in order to determine the mutational load (CM) of the library of mutated genes of interest from step (b).
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公开(公告)号：EP1404830A2
公开(公告)日：2004-04-07
申请号：EP02743542.9
申请日：2002-05-16
申请人： Proteus
IPC分类号： C12N15/10
CPC分类号： C12N15/1027
摘要： Method of preparing fragments for shuffling using DNA repair enzymes. Repair enzymes can be used to repair damaged and mismatched residues to reduce level of mutation in fragments, or can themselves create fragments when used without sufficient DNA polymerase. Invention further includes reaction mixtures created by or during the method, sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
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