公开(公告)号：EP4159857A1

公开(公告)日：2023-04-05

申请号：EP22206649.0

申请日：2012-09-25

申请人： QIAGEN GmbH

发明人： HORLITZ, Martin Dr. ,  SPRENGER-HAUSSELS, Markus Dr. ,  GRÜNEFELD, Peter ,  NOCON, Annette ,  ERBACHER, Christoph

IPC分类号： C12N15/10

摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:
a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase;
b. separating the solid phase with the bound extracellular nucleic acids;
c. optionally washing the extracellular nucleic acids;
d. optionally eluting extracellular nucleic acids from the solid phase.
The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

公开(公告)号：EP3412773A1

公开(公告)日：2018-12-12

申请号：EP18186343.2

申请日：2012-09-25

申请人： QIAGEN GmbH

发明人： HORLITZ, Martin ,  SPRENGER-HAUSSELS, Markus ,  GRÜNEFELD, Peter ,  NOCON, Annette ,  ERBACHER, Christoph

IPC分类号： C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/101

摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:
a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase;
b. separating the solid phase with the bound extracellular nucleic acids;
c. optionally washing the extracellular nucleic acids;
d. optionally eluting extracellular nucleic acids from the solid phase.
The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

公开(公告)号：EP2761001B1

公开(公告)日：2018-08-01

申请号：EP12770443.5

申请日：2012-09-25

申请人： Qiagen GmbH

发明人： HORLITZ, Martin ,  NOCON, Annette ,  SPRENGER-HAUSSELS, Markus ,  GRÜNEFELD, Peter ,  ERBACHER, Christoph

IPC分类号： C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/101

摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

公开(公告)号：EP2761001A1

公开(公告)日：2014-08-06

申请号：EP12770443.5

申请日：2012-09-25

申请人： Qiagen GmbH

发明人： HORLITZ, Martin ,  NOCON, Annette ,  SPRENGER-HAUSSELS, Markus ,  GRÜNEFELD, Peter ,  ERBACHER, Christoph

IPC分类号： C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/101

摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

摘要翻译： 本发明涉及从样品中分离细胞外核酸的方法，其中所述样品任选地通过将细胞外核酸与携带阴离子交换基团的固相结合而包含以下步骤：将细胞外核酸与 具有第一pH的结合混合物中的固相，其允许细胞外核酸与固相的阴离子交换基团结合; 其中所述样品构成所述结合混合物的体积的至少85％; 用结合的细胞外核酸分离固相; 任选地洗涤细胞外核酸; 任选地从固相洗脱细胞外核酸。 该方法的优点是可以处理大的样品体积，并且可以高产率快速分离细胞外核酸。 该方法特别适用于可自动化处理。