公开(公告)号：EP4371998A1

公开(公告)日：2024-05-22

申请号：EP21953718.0

申请日：2021-08-18

申请人： Qitan Technology Ltd., Chengdu

发明人： LIU, Shaowei ,  HE, Jingxiong ,  LI, Qianwen ,  YUE, Feifei

IPC分类号： C07K14/28 ,  C12N15/31 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： C07K14/195 ,  C07K14/28 ,  C12Q1/6869 ,  G01N33/487

摘要： The present invention belongs to the technical field of characterization of target analyte properties, and particularly provides a mutant of a porin monomer, a protein pore comprising same, and use thereof in the detection of a target analyte, wherein an amino acid of the mutant of the porin monomer comprises mutations at one or more of positions corresponding to A74, P75, G76, N77, A78, T79, N80, and F81 of SEQ ID NO: 1.

公开(公告)号：EP4371997A1

公开(公告)日：2024-05-22

申请号：EP21953717.2

申请日：2021-08-18

申请人： Qitan Technology Ltd., Chengdu

发明人： LIU, Shaowei ,  XIE, Fuli ,  HE, Jingxiong ,  YUE, Feifei ,  LI, Qianwen

IPC分类号： C07K14/21 ,  C12N15/31 ,  C12Q1/6869 ,  C12N1/21 ,  C12N15/70

CPC分类号： C07K14/195 ,  C07K14/21 ,  C12N15/70 ,  C12Q1/6869 ,  C12N15/74

摘要： The present invention belongs to the technical field of characterization of target analyte properties, and particularly provides a mutant of a porin monomer, a protein pore comprising same, and use thereof in the detection of a target analyte, wherein an amino acid of the mutant of the porin monomer comprises a sequence set forth in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, or 50% identity thereto, and the amino acid of the mutant of the porin monomer comprises mutations at one or more positions corresponding to T71, S75, or F76 of SEQ ID NO: 1.