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公开(公告)号：EP2262898B1
公开(公告)日：2018-01-24
申请号：EP09718915.3
申请日：2009-03-06
发明人： POUEYMIROU, William , DECHIARA, Thomas, M. , AUERBACH, Wojtek , ECONOMIDES, Aris, N. , GALE, Nicholas, W. , FRENDEWEY, David , VALENZUELA,David, M.
CPC分类号： A01K67/0271 , A01K67/0275 , A01K2207/12 , A01K2217/00 , A01K2217/05 , A01K2217/07 , A01K2217/15 , A01K2217/203 , A01K2217/30 , A01K2227/105 , A01K2267/02 , C12N5/16 , C12N15/8509 , C12N15/907 , C12N2510/00 , C12N2517/02
摘要： Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator, Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making F0 generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM.
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公开(公告)号：EP1141294B1
公开(公告)日：2005-03-02
申请号：EP99968956.5
申请日：1999-12-23
IPC分类号： C12N15/62 , C12N5/10 , C07K14/515 , C07K14/52
CPC分类号： C07K14/515 , A61K38/00 , C07K14/52 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/73 , C07K2319/75 , C12N15/62
摘要： Novel fusion polypeptide ligands that bind Eph family receptors or the Tie-2 receptor are identified, and methods for making the fusion polypeptide ligands in biologically active form are described. Nucleic acids encoding these novel fusion polypeptide ligands enable production of the fusion polypeptide ligands. The method of making the nucleic acids and the fusion polypeptide ligands is broadly applicable to the production of polypeptide ligands in general, resulting in improved affinity and/or increased activity of the ligand when compared to its native form.
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公开(公告)号：EP0859840A1
公开(公告)日：1998-08-26
申请号：EP96937006.0
申请日：1996-10-25
CPC分类号： C07K14/4702
摘要： A novel ligand (Efl-6) that binds the Elk subfamily of Eph receptors is identified, and methods for making the soluble Elf-6 ligand in biologically active form is described. A cDNA clone encoding this novel protein enables production of the recombinant protein, which is useful to support neuronal and other Eph receptor-bearing cell populations.
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公开(公告)号：EP2262898A1
公开(公告)日：2010-12-22
申请号：EP09718915.3
申请日：2009-03-06
发明人： POUEYMIROU, William , DECHIARA, Thomas, M. , AUERBACH, Wojtek , ECONOMIDES, Aris, N. , GALE, Nicholas, W. , FRENDEWEY, David , VALENZUELA,David, M.
CPC分类号： A01K67/0271 , A01K67/0275 , A01K2207/12 , A01K2217/00 , A01K2217/05 , A01K2217/07 , A01K2217/15 , A01K2217/203 , A01K2217/30 , A01K2227/105 , A01K2267/02 , C12N5/16 , C12N15/8509 , C12N15/907 , C12N2510/00 , C12N2517/02
摘要： Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator, Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making F0 generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM.
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公开(公告)号：EP0859840B1
公开(公告)日：2005-12-28
申请号：EP96937006.3
申请日：1996-10-25
CPC分类号： C07K14/4702
摘要： A novel ligand (Efl-6) that binds the Elk subfamily of Eph receptors is identified, and methods for making the soluble Elf-6 ligand in biologically active form is described. A cDNA clone encoding this novel protein enables production of the recombinant protein, which is useful to support neuronal and other Eph receptor-bearing cell populations.
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公开(公告)号：EP1141294A1
公开(公告)日：2001-10-10
申请号：EP99968956.5
申请日：1999-12-23
CPC分类号： C07K14/515 , A61K38/00 , C07K14/52 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/73 , C07K2319/75 , C12N15/62
摘要： Novel fusion polypeptide ligands that bind Eph family receptors or the Tie-2 receptor are identified, and methods for making the fusion polypeptide ligands in biologically active form are described. Nucleic acids encoding these novel fusion polypeptide ligands enable production of the fusion polypeptide ligands. The method of making the nucleic acids and the fusion polypeptide ligands is broadly applicable to the production of polypeptide ligands in general, resulting in improved affinity and/or increased activity of the ligand when compared to its native form.
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