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    IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE
    4.
    发明公开
    IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 审中-公开
    改良的吡咯喹啉喹啉取代的可溶性葡萄糖脱氢酶突变体

    公开(公告)号：EP3246402A1

    公开(公告)日：2017-11-22

    申请号：EP17151869.9

    申请日：2007-04-11

    申请人： Roche Diabetes Care GmbH ,  F. Hoffmann-La Roche AG

    发明人： BOENITZ-DULAT, Mara ,  BECK, Daniela ,  KRATZSCH, Peter ,  SCHMUCK, Rainer ,  VON DER ELTZ, Herbert

    IPC分类号： C12N9/04

    CPC分类号： C12N9/0006

    摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

    摘要翻译： 本发明公开了PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC1.1.5.2）的突变体，其与麦芽糖相比具有改善的对葡萄糖的特异性，其中第348位苏氨酸被甘氨酸，丙氨酸或丝氨酸取代，其中 所述突变体另外包含用于改善突变体的稳定性的至少一种突变和用于改善突变体对葡萄糖的亲和力的一种或多种突变和/或用于进一步改善突变体的特异性的一种或多种突变 葡萄糖突变体与麦芽糖相比较，并且其中位置348对应于醋酸钙不动杆菌s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因，以及这些s-GDH突变体的不同应用，特别是用于确定样品中葡萄糖的浓度。

    IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE
    5.
    发明公开
    IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 有权
    VERBESSERTE MUTANTE MORANTEN PYRROLOCHINOLIN-CHINON-ABHÄNGIGERLÖSLICHERGLUCOSE-DEHYDROGENASE

    公开(公告)号：EP2010649A1

    公开(公告)日：2009-01-07

    申请号：EP07724148.7

    申请日：2007-04-11

    申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

    发明人： BOENITZ-DULAT, Mara ,  BECK, Daniela ,  KRATZSCH, Peter ,  SCHMUCK, Rainer ,  VON DER ELTZ, Herbert

    IPC分类号： C12N9/04

    CPC分类号： C12N9/0006

    摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

    摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于改善突变体的稳定性的突变和用于改善突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。