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1.发明授权IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 有权改进的突变体吡咯CHINON依赖性可溶性葡萄糖脱氢酶
公开(公告)号：EP2010649B1
公开(公告)日：2017-03-08
申请号：EP07724148.7
申请日：2007-04-11
发明人： BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
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2.发明公开IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 审中-公开改良的吡咯喹啉喹啉取代的可溶性葡萄糖脱氢酶突变体
公开(公告)号：EP3246402A1
公开(公告)日：2017-11-22
申请号：EP17151869.9
申请日：2007-04-11
发明人： BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 本发明公开了PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC1.1.5.2）的突变体，其与麦芽糖相比具有改善的对葡萄糖的特异性，其中第348位苏氨酸被甘氨酸，丙氨酸或丝氨酸取代，其中 所述突变体另外包含用于改善突变体的稳定性的至少一种突变和用于改善突变体对葡萄糖的亲和力的一种或多种突变和/或用于进一步改善突变体的特异性的一种或多种突变 葡萄糖突变体与麦芽糖相比较，并且其中位置348对应于醋酸钙不动杆菌s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因，以及这些s-GDH突变体的不同应用，特别是用于确定样品中葡萄糖的浓度。
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3.发明公开
公开(公告)号：EP1402050A2
公开(公告)日：2004-03-31
申请号：EP02740652.9
申请日：2002-05-27
发明人： HOESS, Eva , MEIER, Thomas , PESTLIN, Gabriele , POPP, Friedrich , REICHERT, Klaus , SCHMUCK, Rainer , SCHNEIDINGER, Bernd , SEIDEL, Christoph , TISCHER, Wilhelm
IPC分类号： C12P21/02 , C12N15/62 , C07K14/16 , C07K14/155
CPC分类号： C07K14/005 , C07K2319/00 , C12N2740/15022 , C12N2740/16122 , C12P21/02
摘要： A process for the production of an antifusogenic peptide of a length of about 10 to 50 amino acids in a prokaryotic host cell, characterized in that, under such conditions that inclusion bodies of said non-fusion antifusogenic peptide or said fusion peptide are formed, a) in said host cell there is expressed a nucleic acid encoding said antifusogenic peptide as a non-fusion peptide or encoding a fusion peptide of a length of about 14 to 70 amino acids consisting of said antifusogenic peptide N-terminally linked to a further peptide of a length of about 4 to 30 amino acids; b) said host cell is cultivated; c) said inclusion bodies are recovered and solubilized; d) in the case of said fusion peptide said antifusogenic peptide is cleaved off from said further peptide; and e) said antifusogenic peptide is isolated.
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公开(公告)号：EP1402050B1
公开(公告)日：2014-10-29
申请号：EP02740652.9
申请日：2002-05-27
发明人： HOESS, Eva , MEIER, Thomas , PESTLIN, Gabriele , POPP, Friedrich , REICHERT, Klaus , SCHMUCK, Rainer , SCHNEIDINGER, Bernd , SEIDEL, Christoph , TISCHER, Wilhelm
IPC分类号： C12P21/02 , C12N15/62 , C07K14/12 , C07K14/135 , C07K14/16
CPC分类号： C07K14/005 , C07K2319/00 , C12N2740/15022 , C12N2740/16122 , C12P21/02
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5.发明授权VARIANTS OF SOLUBLE PYRROLOQUINOLINE QUINONE-DEPENDENT GLUCOSE DEHYDROGENASE 有权可溶性PYRROLOQUINOLIN醌依赖性葡萄糖脱氢酶的变异研究
公开(公告)号：EP1332216B1
公开(公告)日：2011-08-17
申请号：EP01988775.1
申请日：2001-10-20
发明人： KRATZSCH, Peter , SCHMUCK, Rainer , BUNK, Daniela , SHAO, Zhixin , THYM, Detlef , KNAPPE, Wolfgang-Reinhold
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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6.发明公开A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES 审中-公开A“步行通过”以科技求多核苷酸的体外重组
公开(公告)号：EP1404825A2
公开(公告)日：2004-04-07
申请号：EP02747439.4
申请日：2002-06-26
IPC分类号： C12N9/16
CPC分类号： C12N9/0034 , C12N9/16 , C12N15/102
摘要： A simple and efficient method for random in vitro mutagenesis and recombination of polynucleotide sequences based on walk-through with chain-elongating molecules and chain terminating molecules e.g. dNTPs/ddNTPs followed by reassembly and amplification is described. The utility of this method for improving protein structure and/or function is demonstrated by creating novel sarcosine oxidase variants and by recombining human placental alkaline phosphates and calf intestinal alkaline phosphatase for better mutants with improved properties.
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7.发明授权THERMOSTABLE MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE 有权THERMO稳定的突变体吡咯CHINON依赖葡萄糖脱氢酶
公开(公告)号：EP1802748B1
公开(公告)日：2011-06-01
申请号：EP05792997.8
申请日：2005-10-14
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these sGDH mutants, particularly for determining the concentration of glucose in a sample.
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公开(公告)号：EP2118125A1
公开(公告)日：2009-11-18
申请号：EP08716417.4
申请日：2008-03-11
CPC分类号： C07K14/472 , C07K14/005 , C07K2319/00 , C12N2740/16122
摘要： The current invention reports a polypeptide conjugate, wherein the conjugate comprises a first polypeptide selected from the group of polypeptides comprising SEQ ID NO: 01 and fragments thereof, and a second polypeptide selected from the group of antifusogenic peptides.
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公开(公告)号：EP1360284B1
公开(公告)日：2008-07-16
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1360284A2
公开(公告)日：2003-11-12
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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