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公开(公告)号：EP1360284B1
公开(公告)日：2008-07-16
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1360284A2
公开(公告)日：2003-11-12
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1206495B1
公开(公告)日：2007-02-14
申请号：EP00948017.9
申请日：2000-07-31
IPC分类号： C07K19/00 , C07K16/46 , C07K7/06 , C12P21/02 , C07K14/025 , C07K16/00 , C07K16/40 , C12N9/34
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , C07K16/00 , C07K2317/624 , C07K2319/00 , C12N7/00 , C12N2710/22022 , C12N2710/22023
摘要： A chimeric polypeptide consisting of a first and a second polypeptide chain, being chemically linked via 1 to 3 cysteine-based disulfide bridges, characterized in that said first polypeptide chain consists of 1 to 3 cysteines and 4 to 12 basic amino acids preferably selected from the group consisting of arginine, lysine and ornithine and said second polypeptide chain consists of 1 to 3 cysteines and 4 to 12 acidic amino acids selected from the group consisting of glutamate and aspartate, and each of said polypeptide chains is linked at its C- or N-terminus to a biologically active compound, is useful as a multimeric pharmaceutical agent.
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公开(公告)号：EP1206495A1
公开(公告)日：2002-05-22
申请号：EP00948017.9
申请日：2000-07-31
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , C07K16/00 , C07K2317/624 , C07K2319/00 , C12N7/00 , C12N2710/22022 , C12N2710/22023
摘要： A chimeric polypeptide consisting of a first and a second polypeptide chain, being chemically linked via 1 to 3 cysteine-based disulfide bridges, characterized in that said first polypeptide chain consists of 1 to 3 cysteines and 4 to 12 basic amino acids preferably selected from the group consisting of arginine, lysine and ornithine and said second polypeptide chain consists of 1 to 3 cysteines and 4 to 12 acidic amino acids selected from the group consisting of glutamate and aspartate, and each of said polypeptide chains is linked at its C- or N-terminus to a biologically active compound, is useful as a multimeric pharmaceutical agent.
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