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公开(公告)号：EP1360284B1
公开(公告)日：2008-07-16
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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2.发明授权VERFAHREN ZUR SYNTHESE UND SELEKTIVEN BIOKATALYTISCHEN MODIFIZIERUNG VON PEPTIDEN, PEPTIDMIMETIKA UND PROTEINEN 有权METHOD肽，肽模拟物和蛋白质的合成和选择性的生物催化修饰
公开(公告)号：EP1534734B1
公开(公告)日：2008-05-07
申请号：EP03794978.1
申请日：2003-09-01
IPC分类号： C07K1/00
CPC分类号： C07K1/12
摘要： The invention relates to a method for the synthesis of peptides, peptide mimetics and/or proteins and/or for the selective N-terminal modification of peptides, peptide mimetics and/or proteins. Said method comprises the following steps: a) an amino constituent comprising at least one amino acid is prepared, b) a carboxy constituent comprising a leaving group on the carboxyl group is prepared, said carboxy constituent being a compound comprising at least one amino acid or a compound comprising at least one marker group or reporter group, and c) the amino constituent and the carboxy constituent are reacted in a reaction medium comprising at least one ionic liquid, in the presence of a protease, a peptidase and/or a hydrolase, a peptide bond being formed between the amino constituent and the carboxy constituent, splitting off the leaving group.
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公开(公告)号：EP1360284A2
公开(公告)日：2003-11-12
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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4.发明公开VERFAHREN ZUR SYNTHESE UND SELEKTIVEN BIOKATALYTISCHEN MODIFIZIERUNG VON PEPTIDEN, PEPTIDMIMETIKA UND PROTEINEN 有权METHOD肽，肽模拟物和蛋白质的合成和选择性的生物催化修饰
公开(公告)号：EP1534734A1
公开(公告)日：2005-06-01
申请号：EP03794978.1
申请日：2003-09-01
IPC分类号： C07K1/00
CPC分类号： C07K1/12
摘要： The invention relates to a method for the synthesis of peptides, peptide mimetics and/or proteins and/or for the selective N-terminal modification of peptides, peptide mimetics and/or proteins. Said method comprises the following steps: a) an amino constituent comprising at least one amino acid is prepared, b) a carboxy constituent comprising a leaving group on the carboxyl group is prepared, said carboxy constituent being a compound comprising at least one amino acid or a compound comprising at least one marker group or reporter group, and c) the amino constituent and the carboxy constituent are reacted in a reaction medium comprising at least one ionic liquid, in the presence of a protease, a peptidase and/or a hydrolase, a peptide bond being formed between the amino constituent and the carboxy constituent, splitting off the leaving group.
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公开(公告)号：EP1206495B1
公开(公告)日：2007-02-14
申请号：EP00948017.9
申请日：2000-07-31
IPC分类号： C07K19/00 , C07K16/46 , C07K7/06 , C12P21/02 , C07K14/025 , C07K16/00 , C07K16/40 , C12N9/34
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , C07K16/00 , C07K2317/624 , C07K2319/00 , C12N7/00 , C12N2710/22022 , C12N2710/22023
摘要： A chimeric polypeptide consisting of a first and a second polypeptide chain, being chemically linked via 1 to 3 cysteine-based disulfide bridges, characterized in that said first polypeptide chain consists of 1 to 3 cysteines and 4 to 12 basic amino acids preferably selected from the group consisting of arginine, lysine and ornithine and said second polypeptide chain consists of 1 to 3 cysteines and 4 to 12 acidic amino acids selected from the group consisting of glutamate and aspartate, and each of said polypeptide chains is linked at its C- or N-terminus to a biologically active compound, is useful as a multimeric pharmaceutical agent.
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公开(公告)号：EP1206495A1
公开(公告)日：2002-05-22
申请号：EP00948017.9
申请日：2000-07-31
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , C07K16/00 , C07K2317/624 , C07K2319/00 , C12N7/00 , C12N2710/22022 , C12N2710/22023
摘要： A chimeric polypeptide consisting of a first and a second polypeptide chain, being chemically linked via 1 to 3 cysteine-based disulfide bridges, characterized in that said first polypeptide chain consists of 1 to 3 cysteines and 4 to 12 basic amino acids preferably selected from the group consisting of arginine, lysine and ornithine and said second polypeptide chain consists of 1 to 3 cysteines and 4 to 12 acidic amino acids selected from the group consisting of glutamate and aspartate, and each of said polypeptide chains is linked at its C- or N-terminus to a biologically active compound, is useful as a multimeric pharmaceutical agent.
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公开(公告)号：EP0674525B1
公开(公告)日：2001-07-25
申请号：EP94903779.0
申请日：1993-12-15
CPC分类号： A61K47/02 , A61K9/0019 , A61K38/193 , A61K47/12
摘要： The invention concerns aqueous pharmaceutical preparations of G-CSF which are stable on storage and which contain at least one buffer selected from the group comprising acetic acid, lactic acid, citric acid, maleic acid, phosphoric acid and their salts or arginine and its salts at a concentration of up to 100 nMol/l.
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