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公开(公告)号：EP1360284B1
公开(公告)日：2008-07-16
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1360284A2
公开(公告)日：2003-11-12
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1399568A2
公开(公告)日：2004-03-24
申请号：EP02716713.9
申请日：2002-02-01
发明人： MÜLLER, Rainer , GLASER, Stephan , GEIPEL, Frank , THALHOFER, Johann-Peter , REXER, Bernhard , SCHNEIDER, Claus , RATKA, Michael , RONNING, Stephanie , ECKSTEIN, Hellmut , GIESSEL, Claudia
CPC分类号： C12N9/6427
摘要： The invention relates to a method for producing recombinant trypsin from the pancreas of a pig which is soluble in Pichia pastoris and secreted into the culture medium, whereby expression at pH 3,0 - 4,0 substantially prevents activation of trypsinogen into β trypsin and the autolysis of β trypsin by trypsin into⊂ trypsin and from there into inactive peptides.
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公开(公告)号：EP1399568B1
公开(公告)日：2005-11-16
申请号：EP02716713.9
申请日：2002-02-01
发明人： MÜLLER, Rainer , GLASER, Stephan , GEIPEL, Frank , THALHOFER, Johann-Peter , REXER, Bernhard , SCHNEIDER, Claus , RATKA, Michael , RONNING, Stephanie , ECKSTEIN, Hellmut , GIESSEL, Claudia
CPC分类号： C12N9/6427
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公开(公告)号：EP1735437B1
公开(公告)日：2011-06-29
申请号：EP05716461.8
申请日：2005-03-31
CPC分类号： C12N9/58 , C11D3/38609
摘要： A solid proteinase K preparation insoluble in water and obtainable by adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, and isolating the precipitated proteinase K has a high purity.
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公开(公告)号：EP1735437A1
公开(公告)日：2006-12-27
申请号：EP05716461.8
申请日：2005-03-31
CPC分类号： C12N9/58 , C11D3/38609
摘要： A solid proteinase K preparation insoluble in water and obtainable by adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, and isolating the precipitated proteinase K has a high purity.
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