公开(公告)号：EP2419530A1

公开(公告)日：2012-02-22

申请号：EP10714201.0

申请日：2010-04-15

申请人： Roche Diagnostics GmbH ,  F.Hoffmann-La Roche AG

发明人： BENTLEY, Gordon ,  ERLICH, Henry, A. ,  HIGUCHI, Russell, Gene ,  HOLCOMB, Cherie

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2535/122

摘要： A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci..

公开(公告)号：EP2203567B1

公开(公告)日：2011-05-11

申请号：EP08839669.2

申请日：2008-10-16

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  ERLICH, Henry A. ,  BENTLEY, Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6855 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/179 ,  C12Q2525/149 ,  C12Q2565/537

摘要： The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.

公开(公告)号：EP2203567A1

公开(公告)日：2010-07-07

申请号：EP08839669.2

申请日：2008-10-16

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  ERLICH, Henry A. ,  BENTLEY, Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6855 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/179 ,  C12Q2525/149 ,  C12Q2565/537

摘要： The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.

公开(公告)号：EP1618210B1

公开(公告)日：2010-09-01

申请号：EP04728336.1

申请日：2004-04-20

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  PELTZ, Gary, Allen ,  FIJAL, Bonnie ,  RO, Sunhee, Kwon ,  LI, Jia

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/156

摘要： Methods and reagents for determining sequence variants present at the FRZB locus, which facilitate identifying individuals at risk for obesity and/or osteoporosis, are provided. In particular, methods for detecting an individual's risk for obesity and/or osteoporosis by detecting at least one polymorphism (e.g., at least one single nucleotide polymorphism) in the FRZB gene in a nucleic acid sample from the individual are provided. Related kits, arrays and systems are also described.

公开(公告)号：EP1618210A1

公开(公告)日：2006-01-25

申请号：EP04728336.1

申请日：2004-04-20

申请人： Roche Diagnostics GmbH ,  F. HOFFMANN-LA ROCHE AG

发明人： HIGUCHI, Russell, Gene ,  PELTZ, Gary, Allen ,  FIJAL, Bonnie ,  RO, Sunhee, Kwon ,  LI, Jia

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/156

摘要： Methods and reagents for determining sequence variants present at the FRZB locus, which facilitate identifying individuals at risk for obesity and/or osteoporosis, are provided. In particular, methods for detecting an individual's risk for obesity and/or osteoporosis by detecting at least one polymorphism (e.g., at least one single nucleotide polymorphism) in the FRZB gene in a nucleic acid sample from the individual are provided. Related kits, arrays and systems are also described.