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1.发明公开VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYOTISCHER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CPG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET 有权富集和/或原核分离DNA由蛋白质的方式方法，它不会甲基化CpG基序含有DNA的特异性结合
公开(公告)号：EP1723233A1
公开(公告)日：2006-11-22
申请号：EP05715667.1
申请日：2005-03-02
申请人： SIRS-Lab GmbH
发明人： SCHMIDT, Karl-Hermann , STRAUBE, Eberhard , RUSSWURM, Stefan , DEIGNER, Hans-Peter , SACHSE, Svea , LEHMANN, Marc
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12N15/1003
摘要： The invention relates to a method for separating and/or enriching prokaryotic DNA, comprising the following steps: a) contacting of at least one prokaryotic DNA that is in solution with a protein that bonds specifically to prokaryotic DNA, said protein being 25 %-35 % homologous with the wild-type CGPB protein, thus forming a protein-DNA complex; and b) separation of the complex. The invention also relates to a kit for carrying out said method.
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2.发明授权VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYOTISCHER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CPG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET 有权富集和/或原核分离DNA由蛋白质的方式方法，它不会甲基化CpG基序含有DNA的特异性结合
公开(公告)号：EP1723233B1
公开(公告)日：2012-04-18
申请号：EP05715667.1
申请日：2005-03-02
申请人： SIRS-Lab GmbH
发明人： SCHMIDT, Karl-Hermann , STRAUBE, Eberhard , RUSSWURM, Stefan , DEIGNER, Hans-Peter , SACHSE, Svea , LEHMANN, Marc
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12N15/1003
摘要： The invention relates to a method for separating and/or enriching prokaryotic DNA, comprising the following steps: a) contacting of at least one prokaryotic DNA that is in solution with a protein that bonds specifically to prokaryotic DNA, said protein being 25 %-35 % homologous with the wild-type CGPB protein, thus forming a protein-DNA complex; and b) separation of the complex. The invention also relates to a kit for carrying out said method.
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