公开(公告)号：EP0209996A3

公开(公告)日：1987-09-02

申请号：EP86304945

申请日：1986-06-25

申请人： SISKA DIAGNOSTICS,INC.

发明人： Musso, Gary Fred ,  Ghosh, Soumitra Shankar ,  Orgel, Leslie Eleazer ,  Wahl, Geoffrey Myles ,  Kaiser, Emil Thomas

IPC分类号： C12Q01/68 ,  C07C143/48 ,  C07D317/30 ,  C07D311/82 ,  C07D319/06 ,  C07D407/12 ,  C07D285/12 ,  C07H21/00 ,  C07D417/12 ,  C07F07/18 ,  C07D495/04 ,  C07D333/00 ,  C07D235/00

CPC分类号： C07H21/00 ,  C07F7/1852 ,  C07F7/1856 ,  C12Q1/6816

摘要： Nucleic acid hybridization probes are provided which comprise an N 4 -(substituted amino) cytosine moiety, wherein the substituted amino group comprises a tag moiety, whereby the probe is detected. Methods of preparing probes of the Invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. Typical tag moieties employed with the probes of the invention are biotinyl, aminothiadiazole and fluorescein derivatives, connected to N 4 -amino groups of modified cytosines of the probe through linker moieties.

摘要翻译： 提供了包含N 4 - （取代氨基）胞嘧啶部分的核酸杂交探针，其中取代的氨基包含标签部分，从而检测探针。 还提供了制备本发明的探针的方法，在这些方法中使用的中间体以及在杂交测定中使用本发明的探针的方法。 与本发明的探针一起使用的典型标签部分是通过接头部分与探针的修饰胞嘧啶的N 4 - 氨基连接的生物素基，氨基噻二唑和荧光素衍生物。

公开(公告)号：EP0209996A2

公开(公告)日：1987-01-28

申请号：EP86304945.8

申请日：1986-06-25

申请人： SISKA DIAGNOSTICS,INC.

发明人： Musso, Gary Fred ,  Ghosh, Soumitra Shankar ,  Orgel, Leslie Eleazer ,  Wahl, Geoffrey Myles ,  Kaiser, Emil Thomas

IPC分类号： C12Q1/68 ,  C07C143/48 ,  C07D317/30 ,  C07D311/82 ,  C07D319/06 ,  C07D407/12 ,  C07D285/12 ,  C07H21/00 ,  C07D417/12 ,  C07F7/18 ,  C07D495/04

CPC分类号： C07H21/00 ,  C07F7/1852 ,  C07F7/1856 ,  C12Q1/6816

摘要： Nucleic acid hybridization probes are provided which comprise an N 4 -(substituted amino) cytosine moiety, wherein the substituted amino group comprises a tag moiety, whereby the probe is detected. Methods of preparing probes of the Invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. Typical tag moieties employed with the probes of the invention are biotinyl, aminothiadiazole and fluorescein derivatives, connected to N 4 -amino groups of modified cytosines of the probe through linker moieties.

摘要翻译： 提供了包含N 4 - （取代氨基）胞嘧啶部分的核酸杂交探针，其中取代的氨基包含标签部分，由此检测探针。 还提供了制备本发明的探针的方法，在这些方法中使用的中间体以及在杂交测定中使用本发明的探针的方法。 与本发明的探针一起使用的典型标签部分是通过接头部分与探针的修饰胞嘧啶的N 4 - 氨基连接的生物素基，氨基噻二唑和荧光素衍生物。

公开(公告)号：EP0210021A3

公开(公告)日：1987-09-02

申请号：EP86305300

申请日：1986-07-09

申请人： SISKA DIAGNOSTICS,INC.

发明人： Kaiser, Emil Thomas ,  Musso, Gary Fred ,  Ghosh, Soumitra Shankar ,  Orgel, Leslie Eleazer ,  Wahl, Geoffrey Myles

IPC分类号： C12Q01/68 ,  C07H19/20 ,  C07H19/10 ,  C07C143/78 ,  C07D317/30 ,  C07D319/06 ,  C07D285/12 ,  C07D417/12

CPC分类号： G01N33/535 ,  C07F7/1852 ,  C07F7/1856 ,  C07H21/00 ,  G01N33/532

摘要： Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosphates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or heteropolymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then detecting the bound reporter group, as by production of a fluorescent or colored product in a reaction catalyzed by an enzyme component of the reporter group. Also provided are en- z y me immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.

公开(公告)号：EP0210021A2

公开(公告)日：1987-01-28

申请号：EP86305300.5

申请日：1986-07-09

申请人： SISKA DIAGNOSTICS,INC.

发明人： Kaiser, Emil Thomas ,  Musso, Gary Fred ,  Ghosh, Soumitra Shankar ,  Orgel, Leslie Eleazer ,  Wahl, Geoffrey Myles

IPC分类号： C12Q1/68 ,  C07H19/20 ,  C07H19/10 ,  C07C143/78 ,  C07D317/30 ,  C07D319/06 ,  C07D285/12 ,  C07D417/12

CPC分类号： G01N33/535 ,  C07F7/1852 ,  C07F7/1856 ,  C07H21/00 ,  G01N33/532

摘要： Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosphates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or heteropolymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then detecting the bound reporter group, as by production of a fluorescent or colored product in a reaction catalyzed by an enzyme component of the reporter group. Also provided are en- z y me immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.

摘要翻译： 提供核酸杂交探针，其包含与碳酸酐酶的芳族磺酰胺抑制剂化学连接的核苷碱基或末端核苷酸磷酸酯。 还提供了制备本发明的探针的方法，在这些方法中使用的中间体以及在杂交测定中使用本发明的探针的方法。 本发明的探针通过结合报告基团如酶的均聚物或异源聚合酶来检测，所述报告基团包括结合与探针连接的抑制剂的碳酸酐酶，然后通过生产检测结合的报告基团 的荧光或有色产物在由报道基团的酶组分催化的反应中。 还提供了酶免疫测定，其中抗体的检测是通过包含由碳酸酐酶催化的化学反应的方法。