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    Mitochondrial P450
    1.
    发明公开
    Mitochondrial P450 失效
    线粒体P450。

    公开(公告)号：EP0477961A2

    公开(公告)日：1992-04-01

    申请号：EP91116485.3

    申请日：1991-09-26

    申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

    发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

    IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

    CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

    摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome.
    An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5β-cholestane-3α, 7α, 12α, 27-tetrol, 25-hydroxyvitamin D₃ and 1α,25-dihydroxyvitamin D₃ are also disclosed.

    摘要翻译： 公开了线粒体P450在酵母中的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N末端的信号序列已被酵母线粒体蛋白的信号和嵌合P450取代，其中后一信号被靶向进一步取代 信号序列到微粒体。 用于大规模生产酶的表达质粒，携带表达质粒的重组酵母菌株，使用重组酵母生产酶的方法，以及生产5β-胆甾烷-3α，7α ，12α，27-四醇，25-羟基维生素D3和1α，25-二羟基维生素D3。

    Mitochondrial P450
    3.
    发明公开
    Mitochondrial P450 失效
    线粒体P450

    公开(公告)号：EP0477961A3

    公开(公告)日：1992-04-29

    申请号：EP91116485.3

    申请日：1991-09-26

    申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

    发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

    IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

    CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

    摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome. An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5β-cholestane-3α, 7α, 12α, 27-tetrol, 25-hydroxyvitamin D₃ and 1α,25-dihydroxyvitamin D₃ are also disclosed.

    摘要翻译： 披露了酵母中线粒体P450的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N端的信号序列已经被酵母线粒体蛋白的信号和嵌合P450置换，其中后者信号已被靶向物进一步取代 信号序列转化为微粒体。 用于大规模生产该酶的表达质粒，携带该表达质粒的重组酵母菌株，使用该重组酵母生产该酶的方法以及用于生产5β-胆甾烷-3α，7α，12α， 27-四醇，25-羟基维生素D 3和1α，25-二羟基维生素D 3。