公开(公告)号：EP0649472A1

公开(公告)日：1995-04-26

申请号：EP93916837.0

申请日：1993-06-29

申请人： BOARD OF REGENTS THE UNIVERSITY OF TEXAS SYSTEM

发明人： FISHER, Charles, W. ,  BARNES, Henry, J. ,  ESTABROOK, Ronald, W.

IPC分类号： C12N1 ,  C12N9 ,  C12N15

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C12N9/0085 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/99009

摘要： Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P45017α- hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17α-hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria.

公开(公告)号：EP2828288A1

公开(公告)日：2015-01-28

申请号：EP13714173.5

申请日：2013-03-21

申请人： E.J. Squires Ltd. ,  Genus Plc ,  Squires, E. James

发明人： SQUIRES, E. James

IPC分类号： C07K14/435

CPC分类号： C12N9/0085 ,  A01K67/0275 ,  A01K2227/108 ,  C12N9/004 ,  C12N15/8509 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Y106/02002 ,  C12Y401/0203

摘要： Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.

公开(公告)号：EP0649472B1

公开(公告)日：2003-12-10

申请号：EP93916837.3

申请日：1993-06-29

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： FISHER, Charles, W. ,  BARNES, Henry, J. ,  ESTABROOK, Ronald, W.

IPC分类号： C12N15/62 ,  C12N15/11 ,  C12N15/12 ,  C12N15/31 ,  C12N15/33 ,  C12N15/67 ,  C12N15/70 ,  C12P33/00 ,  C12N9/02

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C12N9/0085 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/99009

摘要： Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P45017α- hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17α-hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria.

公开(公告)号：EP0477961B1

公开(公告)日：1996-09-11

申请号：EP91116485.3

申请日：1991-09-26

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

公开(公告)号：EP0477961A3

公开(公告)日：1992-04-29

申请号：EP91116485.3

申请日：1991-09-26

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome. An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5β-cholestane-3α, 7α, 12α, 27-tetrol, 25-hydroxyvitamin D₃ and 1α,25-dihydroxyvitamin D₃ are also disclosed.

摘要翻译： 披露了酵母中线粒体P450的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N端的信号序列已经被酵母线粒体蛋白的信号和嵌合P450置换，其中后者信号已被靶向物进一步取代 信号序列转化为微粒体。 用于大规模生产该酶的表达质粒，携带该表达质粒的重组酵母菌株，使用该重组酵母生产该酶的方法以及用于生产5β-胆甾烷-3α，7α，12α， 27-四醇，25-羟基维生素D 3和1α，25-二羟基维生素D 3。

公开(公告)号：EP2645847B1

公开(公告)日：2018-01-17

申请号：EP11793960.3

申请日：2011-11-29

申请人： Massachusetts Institute of Technology

发明人： STEPHANOPOULOS, Gregory ,  AJIKUMAR, Parayil, K.

IPC分类号： A01H5/00 ,  A01H13/00 ,  C12N15/82 ,  A23L2/60

CPC分类号： C12N15/8243 ,  A01H5/00 ,  A01H13/00 ,  A23L2/60 ,  A23L27/36 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0085 ,  C12N9/1085 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12P7/42 ,  C12P15/00 ,  C12P17/02 ,  C12Y106/02004 ,  C12Y114/13 ,  C12Y114/13076 ,  C12Y114/13078 ,  C12Y114/99009 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012 ,  C12Y505/01013 ,  Y02P20/52

摘要： The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.

公开(公告)号：EP2645847A1

公开(公告)日：2013-10-09

申请号：EP11793960.3

申请日：2011-11-29

申请人： Massachusetts Institute Of Technology

发明人： STEPHANOPOULOS, Gregory ,  AJIKUMAR, Parayil, K.

IPC分类号： A01H5/00 ,  A01H13/00 ,  C12N15/82 ,  A23L1/236 ,  A23L2/60

CPC分类号： C12N15/8243 ,  A01H5/00 ,  A01H13/00 ,  A23L2/60 ,  A23L27/36 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0085 ,  C12N9/1085 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12P7/42 ,  C12P15/00 ,  C12P17/02 ,  C12Y106/02004 ,  C12Y114/13 ,  C12Y114/13076 ,  C12Y114/13078 ,  C12Y114/99009 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012 ,  C12Y505/01013 ,  Y02P20/52

摘要： The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.

摘要翻译： 本发明涉及细胞中甜菊醇或甜菊糖苷生物合成途径酶的重组表达以及甜菊醇或甜菊糖苷的生产。

公开(公告)号：EP0649472A4

公开(公告)日：1997-03-19

申请号：EP93916837

申请日：1993-06-29

申请人： UNIV TEXAS

发明人： FISHER CHARLES W ,  BARNES HENRY J ,  ESTABROOK RONALD W

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12N15/67 ,  C12N15/70 ,  C12N15/62 ,  C12N15/11 ,  C12N15/12 ,  C12N15/31 ,  C12N15/33 ,  C12P33/00

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C12N9/0085 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/99009

公开(公告)号：EP0477961A2

公开(公告)日：1992-04-01

申请号：EP91116485.3

申请日：1991-09-26

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome.
An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5β-cholestane-3α, 7α, 12α, 27-tetrol, 25-hydroxyvitamin D₃ and 1α,25-dihydroxyvitamin D₃ are also disclosed.

摘要翻译： 公开了线粒体P450在酵母中的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N末端的信号序列已被酵母线粒体蛋白的信号和嵌合P450取代，其中后一信号被靶向进一步取代 信号序列到微粒体。 用于大规模生产酶的表达质粒，携带表达质粒的重组酵母菌株，使用重组酵母生产酶的方法，以及生产5β-胆甾烷-3α，7α ，12α，27-四醇，25-羟基维生素D3和1α，25-二羟基维生素D3。