公开(公告)号：EP1710579A2

公开(公告)日：2006-10-11

申请号：EP06006378.1

申请日：2006-03-28

申请人： SYSMEX CORPORATION

发明人： Mori, Yusuke ,  Mizukami, Toshihiro ,  Tsuji, Tomohiro ,  Ikeuchi, Yoshiro

IPC分类号： G01N33/50 ,  G01N33/80 ,  G01N15/14 ,  G01N21/64 ,  G01N15/02

CPC分类号： G01N15/147 ,  G01N33/5002 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0073

摘要： A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.

摘要翻译： 描述了一种用于计数样本中的巨核细胞的方法。 在该方法中，首先将样品中的红细胞裂解，并用荧光染料染色巨核细胞中的核酸，由此制备测定样品。 接下来，用激发光照射测量样品中的单元，从而检测从单元发射的前向散射光，侧向散射光和荧光。 基于检测到的前向散射光，荧光和侧向散射光来鉴定巨核细胞。 然后，计算鉴定的巨核细胞。

公开(公告)号：EP1574839A1

公开(公告)日：2005-09-14

申请号：EP05005237.2

申请日：2005-03-10

申请人： SYSMEX CORPORATION

发明人： Ikeuchi, Yoshiro ,  Oguni, Shinichiro ,  Fukuma, Daigo ,  Imazu, Masanori ,  Lissen, Jo

IPC分类号： G01N15/14

CPC分类号： G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  Y10T436/101666

摘要： A sample analyzer including a detection unit for irradiating a biological sample with light and obtaining optical information; a cell classification processor for classifying cells contained in the biological sample into cell groups based on the optical information; a scattered light information obtaining processor for obtaining scattered light information relating to a cell included in a predetermined cell group; a calculation processor for calculating a component value corresponding to an amount of component contained in cell included in the predetermined cell group based on the scattered light information; and an output device for outputting the component value calculated by the calculation means is disclosed. A computer program product is also disclosed.

摘要翻译： 一种样本分析器，包括用于用光照射生物样品并获得光学信息的检测单元; 细胞分类处理器，用于基于所述光学信息将生物样品中包含的细胞分类成细胞组; 散射光信息获取处理器，用于获得与包括在预定单元组中的单元有关的散射光信息; 计算处理器，用于基于散射光信息计算与包含在所述预定单元组中的单元中包含的分量的量相对应的分量值; 并且公开了一种用于输出由计算装置计算出的分量值的输出装置。 还公开了一种计算机程序产品。

公开(公告)号：EP1203825A2

公开(公告)日：2002-05-08

申请号：EP01125418.2

申请日：2001-10-31

申请人： Sysmex Corporation

发明人： Sakai, Yasuhiro ,  Kawashima, Yasuyuki ,  Inoue, Junya ,  Ikeuchi, Yoshiro

IPC分类号： C12Q1/04 ,  G01N1/30

CPC分类号： G01N1/30 ,  C12Q1/04 ,  G01N2001/302 ,  Y10T436/108331

摘要： A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.

摘要翻译： 染色细菌的方法包括：在能够降低亚硝酸根离子染色细菌样品中的物质的存在的样品工作一个聚甲炔染料。 检测细菌的方法，包括以下步骤：（1）通过以下方法工作的一个样本一个聚甲炔染料作为样品中如上所述染色的细菌，（2）将这样处理过的样品到流动的检测部血细胞计数 和照射染色的细菌细胞一个个光来测量散射光以及荧光的光从每个单元发射; 和（3）上的散射光信号的强度和到荧光信号或脉冲宽度反射颗粒的长度来计算细菌的强鉴别从其他组件细菌在雅舞蹈。

公开(公告)号：EP1203825B1

公开(公告)日：2005-09-21

申请号：EP01125418.2

申请日：2001-10-31

申请人： SYSMEX CORPORATION

发明人： Sakai, Yasuhiro ,  Kawashima, Yasuyuki ,  Inoue, Junya ,  Ikeuchi, Yoshiro

IPC分类号： C12Q1/04 ,  C12Q1/06 ,  G01N1/30

CPC分类号： G01N1/30 ,  C12Q1/04 ,  G01N2001/302 ,  Y10T436/108331

公开(公告)号：EP1004880A3

公开(公告)日：2003-02-05

申请号：EP98310004.1

申请日：1998-12-07

申请人： Sysmex Corporation

发明人： Tsuji, Tomohiro ,  Sakata, Takashi ,  Ikeuchi, Yoshiro ,  Oguni, Shin'ichiro

IPC分类号： G01N33/50 ,  G01N33/58 ,  G01N33/52 ,  G01N33/80

CPC分类号： G01N33/5094 ,  G01N15/1459 ,  G01N33/80 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10T436/101666 ,  Y10T436/107497

摘要： Reagents and a method for simple and rapid discrimination and counting of erythroblasts in peripheral blood or circulatory system-related samples accurately with high precision is disclosed. The reagents include a hemolytic agent for dissolving erythrocytes in a body fluid sample and for conditioning leukocytes and erythroblasts in the sample to be suitable for staining, and including at least one fluorescent dye selected to stain leukocytes and erythroblasts differentially. When the selected fluorescent dye is mixed with the sample, a detectable difference in fluorescence intensity at least between leukocytes and erythroblasts arises under laser illumination in flow cytometric analysis. The reagents further include surfactant added to the hemolytic agent, selected to enable flow cytometric discrimination of erythroblasts in the body fluid sample by their maturation stages.

公开(公告)号：EP1710579B1

公开(公告)日：2014-07-30

申请号：EP06006378.1

申请日：2006-03-28

申请人： SYSMEX CORPORATION

发明人： Mori, Yusuke ,  Mizukami, Toshihiro ,  Tsuji, Tomohiro ,  Ikeuchi, Yoshiro

IPC分类号： G01N33/50 ,  G01N33/80 ,  G01N15/14 ,  G01N21/64 ,  G01N15/02

CPC分类号： G01N15/147 ,  G01N33/5002 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0073

公开(公告)号：EP1887357A2

公开(公告)日：2008-02-13

申请号：EP07012709.7

申请日：2007-06-28

申请人： SYSMEX CORPORATION

发明人： Uchihashi, Kinya ,  Ikeuchi, Yoshiro ,  Narisada, Noriyuki

IPC分类号： G01N33/50 ,  G01N15/14 ,  G01N33/49

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1486 ,  G01N2015/1497 ,  Y10T436/10 ,  Y10T436/101666

摘要： The present invention is to present a blood analyzer which can prevent an abnormal number of white blood cells caused by the lipid particles or the red blood cells of poor hemolysis from being output. The blood analyzer comprises: first white blood cell number obtaining means; second white blood cell number obtaining means; output means for outputting the number of the white blood cells obtained by the second white blood cell number obtaining means, and determining means for determining whether the number of the white blood cells obtained by the second white blood cell number obtaining means is abnormal, wherein the output means is configured to output the number of the white blood cells obtained by the first white blood cell number obtaining means when the determining means determines that the number of the white blood cells obtained by the second white blood cell number obtaining means is abnormal.

摘要翻译： 本发明是提供一种可以防止由脂质颗粒引起的异常数量的血液分析仪或者溶血不良的红细胞输出的血液分析装置。 血液分析装置包括：第一白细胞数获取装置; 第二白细胞数获得装置; 用于输出由第二白细胞数获得装置获得的白细胞数的输出装置，以及用于确定由第二白细胞数获得装置获得的白细胞数是否异常的确定装置，其中， 输出装置被配置为当确定装置确定由第二白细胞数获得装置获得的白细胞数异常时，输出由第一白细胞数获得装置获得的白细胞数。

公开(公告)号：EP1405918A1

公开(公告)日：2004-04-07

申请号：EP03022247.5

申请日：2003-10-01

申请人： Sysmex Corporation

发明人： Kawashima, Yasuyuki ,  Ikeuchi, Yoshiro ,  Sakai, Yasuhiro

IPC分类号： C12Q1/06 ,  G01N1/30

CPC分类号： G01N1/30 ,  C12Q1/06 ,  G01N15/14

摘要： Methods for counting bacteria are described that include: (a) preparing an assay sample by staining a specimen using a fluorescent dye, thereby producing a difference in fluorescent intensity between live bacteria and dead bacteria; (b) detecting optical information from the assay sample; and (c) classifying and counting the live bacteria and the dead bacteria based on the detected optical information. Bacteria counting apparatuses and reagent kits for counting bacteria are also described.

摘要翻译： 细菌计数包括通过使用荧光染料染色样品来制备测定样品，以产生活细菌和死细菌之间的荧光强度差; 检测来自测定样品的光学信息; 基于检测到的光学信息对活细菌和死菌进行分类和计数是新的。 还包括以下独立权利要求：（1）细菌计数装置，包括用荧光染料染色试样制备测定样品的测定样品制备单元，从而产生活细菌与死细菌之间的荧光强度差; 检测单元，用于从所述测定样品检测光学信息; 以及分析单元，其基于由检测单元检测的光学信息对活细菌和死菌进行分类和计数; 和（2）用于计数细菌的试剂盒，其包含基本上不含表面活性剂的第一稀释液; 包含表面活性剂的第二稀释液; 和包含荧光染料的污渍。

公开(公告)号：EP0872734B1

公开(公告)日：2001-03-14

申请号：EP98106911.5

申请日：1998-04-16

申请人： Sysmex Corporation

发明人： Uchihashi, Kinya ,  Ikeuchi, Yoshiro ,  Shirakami, Atsushi ,  Hamaguchi, Yukio

IPC分类号： G01N33/50 ,  G01N33/72

CPC分类号： G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525

公开(公告)号：EP1004880A2

公开(公告)日：2000-05-31

申请号：EP98310004.1

申请日：1998-12-07

申请人： Sysmex Corporation

发明人： Tsuji, Tomohiro ,  Sakata, Takashi ,  Ikeuchi, Yoshiro ,  Oguni, Shin'ichiro

IPC分类号： G01N33/50 ,  G01N33/58 ,  G01N33/52

CPC分类号： G01N33/5094 ,  G01N15/1459 ,  G01N33/80 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10T436/101666 ,  Y10T436/107497

摘要： Reagents and a method for simple and rapid discrimination and counting of erythroblasts in peripheral blood or circulatory system-related samples accurately with high precision is disclosed. The reagents include a hemolytic agent for dissolving erythrocytes in a body fluid sample and for conditioning leukocytes and erythroblasts in the sample to be suitable for staining, and including at least one fluorescent dye selected to stain leukocytes and erythroblasts differentially. When the selected fluorescent dye is mixed with the sample, a detectable difference in fluorescence intensity at least between leukocytes and erythroblasts arises under laser illumination in flow cytometric analysis. The reagents further include surfactant added to the hemolytic agent, selected to enable flow cytometric discrimination of erythroblasts in the body fluid sample by their maturation stages.

摘要翻译： 公开了一种以高精度准确地对外周血或循环系统相关样品中的红细胞进行简单快速辨别和计数的方法。 试剂包括用于将红细胞溶解在体液样品中并用于调节样品中适合染色的白细胞和成红细胞的溶血剂，并且包括至少一种选择用于染色白细胞和成红细胞的荧光染料。 当选择的荧光染料与样品混合时，至少在白细胞和成红细胞之间的荧光强度的可检测差异在流式细胞术分析的激光照射下产生。 试剂还包括添加到溶血剂中的表面活性剂，其被选择用于通过其成熟阶段对体液样品中的红细胞进行流式细胞分辨。