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公开(公告)号:EP2563918A1
公开(公告)日:2013-03-06
申请号:EP11777679.9
申请日:2011-04-25
发明人: CUI, Xiaoxia , DAVIS, Gregory , GREGORY, Philip, D. , HOLMES, Michael, C. , WEINSTEIN, Edward, J.
CPC分类号: C12N9/22 , A01K67/0275 , A01K2207/05 , A01K2217/07 , A01K2227/105 , A01K2267/03 , A61K38/00 , C07K2319/81 , C12N15/102 , C12N15/907
摘要: Disclosed herein are methods and compositions for genome editing of a
Rosa locus, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.摘要翻译: 本文公开了使用包含锌指蛋白和切割结构域或切割半结构域的融合蛋白的用于基因组编辑罗莎基因座的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸,以及包含所述多核苷酸和融合蛋白的细胞。
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2.
公开(公告)号:EP2563918B1
公开(公告)日:2016-11-23
申请号:EP11777679.9
申请日:2011-04-25
发明人: CUI, Xiaoxia , DAVIS, Gregory , GREGORY, Philip, D. , HOLMES, Michael, C. , WEINSTEIN, Edward, J.
IPC分类号: C12N15/90 , A61K38/00 , C12N9/22 , C12N15/10 , A01K67/027
CPC分类号: C12N9/22 , A01K67/0275 , A01K2207/05 , A01K2217/07 , A01K2227/105 , A01K2267/03 , A61K38/00 , C07K2319/81 , C12N15/102 , C12N15/907
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公开(公告)号:EP3222715A1
公开(公告)日:2017-09-27
申请号:EP17165881.8
申请日:2004-08-06
IPC分类号: C12N9/22 , C12N15/62 , C12N15/01 , A61K35/12 , C07K14/715 , C07K14/435 , C07K14/47 , C12N9/14 , C12N15/85 , C12N15/90
CPC分类号: C12N15/10 , C07K14/4702 , C07K2319/00 , C07K2319/81 , C12N9/22 , C12N15/902
摘要: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to M the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
摘要翻译: 本文公开了用于基因组序列的靶向切割,基因组序列的靶向改变以及基因组区域和与基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 该组合物包括含有切割结构域(或切割半结构域)和工程化锌指结构域的融合蛋白以及编码它们的多核苷酸。 用于靶向切割的方法包括将此类融合蛋白或编码它们的多核苷酸引入细胞中。 用于靶向重组的方法另外包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。
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公开(公告)号:EP2281050B1
公开(公告)日:2014-04-02
申请号:EP09734442.8
申请日:2009-04-13
CPC分类号: C12N15/907 , C12N15/63 , C12N15/8213
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公开(公告)号:EP2281050A1
公开(公告)日:2011-02-09
申请号:EP09734442.8
申请日:2009-04-13
CPC分类号: C12N15/907 , C12N15/63 , C12N15/8213
摘要: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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公开(公告)号:EP2764102A1
公开(公告)日:2014-08-13
申请号:EP12838704.0
申请日:2012-10-04
IPC分类号: C12N15/55 , A61K31/7088
CPC分类号: C12N15/85 , A61K31/7088 , A61K31/7105 , A61K31/713 , C07K2/00 , C07K2319/81 , C12N9/16 , C12N15/87 , C12Y301/21004
摘要: Methods and compositions for regulating HIV infection and/or replication in which an anti-HIV transgene is integrated into the genome of a cell using a nuclease.
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公开(公告)号:EP1651660A2
公开(公告)日:2006-05-03
申请号:EP04780272.3
申请日:2004-08-06
IPC分类号: C07H21/04 , C12N9/00 , C12N15/00 , C12N15/09 , C12N15/85 , C12N15/70 , C12N15/63 , C12Q1/68 , A61K35/00
CPC分类号: C12N15/10 , C07K14/4702 , C07K2319/00 , C07K2319/81 , C12N9/22 , C12N15/902
摘要: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
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