公开(公告)号：EP2771457A2

公开(公告)日：2014-09-03

申请号：EP12844270.4

申请日：2012-10-25

申请人： Sangamo BioSciences, Inc. ,  The Regents of the University of California

发明人： COST, Gregory J. ,  HOLMES, Michael C. ,  KASAHARA, Noriyuki ,  LAGANIERE, Josee ,  MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  URNOV, Fyodor ,  ZHANG, Lei

IPC分类号： C12N9/16 ,  C12N15/00

CPC分类号： C12N15/85 ,  C07K2319/81 ,  C12N9/1077 ,  C12N9/22 ,  C12N9/96 ,  C12N15/907 ,  C12Y204/02008 ,  C12Y301/21004

摘要： Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.

摘要翻译： 使用这些核酸酶修饰HPRT基因座的核酸酶和方法，并增加靶向位点和克隆以及产生动物的基因修饰频率。

公开(公告)号：EP2771457B1

公开(公告)日：2017-11-22

申请号：EP12844270.4

申请日：2012-10-25

申请人： Sangamo Therapeutics, Inc. ,  The Regents of the University of California

发明人： COST, Gregory J. ,  HOLMES, Michael C. ,  KASAHARA, Noriyuki ,  LAGANIERE, Josee ,  MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  URNOV, Fyodor ,  ZHANG, Lei

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/90

CPC分类号： C12N15/85 ,  C07K2319/81 ,  C12N9/1077 ,  C12N9/22 ,  C12N9/96 ,  C12N15/907 ,  C12Y204/02008 ,  C12Y301/21004

摘要： Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.

公开(公告)号：EP1913149A2

公开(公告)日：2008-04-23

申请号：EP06788559.0

申请日：2006-07-26

申请人： Sangamo BioSciences, Inc.

发明人： HOLMES, Michael C. ,  URNOV, Fyodor ,  GREGORY, Philip D. ,  REBAR, Edward J. ,  BRENNAN, Sean M.

IPC分类号： C12P21/06 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/907 ,  A61K48/0008 ,  A61K48/005 ,  A61K48/0058 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/62 ,  C12N15/90

摘要： Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.

公开(公告)号：EP2997146A2

公开(公告)日：2016-03-23

申请号：EP14797155.0

申请日：2014-05-15

申请人： Sangamo BioSciences, Inc.

发明人： CONWAY, Anthony ,  COST, Gregory J. ,  DEKELVER, Russell ,  REBAR, Edward J. ,  REIK, Andreas ,  URNOV, Fyodor ,  WANG, Jianbin ,  ZHANG, H. Steve

IPC分类号： C12N15/11

CPC分类号： C12N15/907 ,  C07K2/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/902 ,  C12N2320/30 ,  C12Y301/21004

摘要： Methods and compositions comprising single guide RNAs targeted to an endogenous mammalian β globin gene (HBB), gamma globin gene (HBG1), B-cell lymphoma/leukemia 11A (BCL11A) gene, Kruppel-like factor 1 (KLF1) gene, CCR5 gene, CXCR4 gene, PPP1R12C (AAVS1) gene, hypoxanthine phosphoribosyltransferase (HPRT) gene, albumin gene, Factor VIII gene, Factor IX gene, Leucine-rich repeat kinase 2 (LRRK2) gene, Hungtingin (Htt) gene, rhodopsin (RHO) gene, Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene, surfactant protein B gene (SFTPB), T-cell receptor alpha (TRAC) gene, T-cell receptor beta (TRBC) gene, programmed cell death 1 (PD1) gene, Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) gene, human leukocyte antigen (HLA) A gene, a LMP7 gene, a Transporter associated with Antigen Processing (TAP) gene, tapasin gene (TAPBP), a class II major histocompatibility complex transactivator (CIITA) gene, a dystrophin gene (DMD), a glucocorticoid receptor gene (GR), IL2RG gene or RFX5 for genetic alteration of cells are provided.

摘要翻译： 提供了用于细胞遗传改变的方法和组合物。

公开(公告)号：EP2596011A2

公开(公告)日：2013-05-29

申请号：EP11810427.2

申请日：2011-07-21

申请人： Sangamo BioSciences, Inc. ,  Board Of Regents, The University Of Texas System

发明人： COLLINGWOOD, Trevor ,  COOPER, Laurence J.,N. ,  GREGORY, Philip D. ,  HOLMES, Michael C. ,  MILLER, Jeffrey C. ,  REBAR, Edward J. ,  REIK, Andreas ,  URNOV, Fyodor

IPC分类号： C07K14/00

CPC分类号： C07K14/70539 ,  A61K35/17 ,  C07K2319/81 ,  C12N15/907 ,  C12N2800/80

摘要： Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.

公开(公告)号：EP3080274A2

公开(公告)日：2016-10-19

申请号：EP14869719.6

申请日：2014-12-09

申请人： Sangamo BioSciences, Inc.

发明人： MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  WECHSLER, Thomas ,  ZHANG, Lei

IPC分类号： C12N15/64 ,  C12N15/66 ,  C12N5/00 ,  C12N5/02

摘要： Disclosed herein are methods and compositions for insertion of transgene sequences encoding proteins involved in clotting into the genome of a cell for treating conditions including hemophilias.

摘要翻译： 本文公开了用于将编码参与凝血的蛋白质的转基因序列插入细胞的基因组中用于治疗包括血友病的病症的方法和组合物。

公开(公告)号：EP3080143A1

公开(公告)日：2016-10-19

申请号：EP14869305.4

申请日：2014-12-09

申请人： Sangamo BioSciences, Inc.

发明人： MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J.

IPC分类号： C07K14/00

CPC分类号： C07K14/47 ,  A61K48/00 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N9/644 ,  C12N15/86 ,  C12N2750/14133 ,  C12N2750/14142 ,  C12N2750/14143

摘要： Disclosed herein are methods and compositions for insertion of transgene sequences encoding proteins involved in clotting into the genome of a cell for treating conditions including hemophilias. In one aspect, described herein is a non-naturally occurring zinc-finger protein (ZFP) that binds to a target site in a region of interest (e.g., an albumin gene) in a genome, wherein the ZFP comprises one or more engineered zinc-finger binding domains. In certain embodiments, the zinc finger domain recognizes a target site in an albumin gene. In some embodiments, the zinc finger protein comprises five or six zinc finger domains designated and ordered F1 to F5 or F1 to F6, as shown in a single row of Table 1.

公开(公告)号：EP3156062A1

公开(公告)日：2017-04-19

申请号：EP16200664.7

申请日：2011-05-17

申请人： Sangamo BioSciences, Inc.

发明人： GREGORY, Philip D. ,  MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  TAN, Siyuan ,  URNOV, Fyodor ,  ZHANG, Lei

IPC分类号： A61K38/00 ,  A61K38/16 ,  C12N15/62 ,  C12N9/22

CPC分类号： C12N15/907 ,  A61K38/00 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/52 ,  C12N15/62 ,  C12N15/8213 ,  C12N15/85 ,  C12Y301/21004

摘要： Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.

摘要翻译： 本文公开了编码包含新的DNA结合结构域（包括TALE DNA结合域）的细胞和生物体的多肽，多核苷酸。 还公开了使用这些新的DNA结合结构域来调节内源细胞序列的基因表达和/或基因组编辑的方法。

公开(公告)号：EP2890780A2

公开(公告)日：2015-07-08

申请号：EP13833658.1

申请日：2013-08-29

申请人： Sangamo BioSciences, Inc.

发明人： COST, Gregory J. ,  GREGORY, Philip D. ,  GUSCHIN, Dmitry ,  HOLMES, Michael C. ,  MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  REIK, Andreas ,  URNOV, Fyodor ,  ZHANG, Lei

IPC分类号： C12N5/071

摘要： Disclosed herein are nucleases and methods of using these nucleases for alteration of a globin gene and generation of cells.

公开(公告)号：EP2780460A1

公开(公告)日：2014-09-24

申请号：EP12850371.1

申请日：2012-11-16

申请人： Sangamo BioSciences, Inc.

发明人： GREGORY, Philip D. ,  MILLER, Jeffrey C. ,  PASCHON, David ,  REBAR, Edward J. ,  TAN, Siyuan ,  URNOV, Fyodor ,  ZHANG, Lei

IPC分类号： C12N15/87 ,  C07K14/195

CPC分类号： C07K14/195 ,  C07K2319/80 ,  C12N1/16 ,  C12N5/04 ,  C12N5/0602 ,  C12N9/1007 ,  C12N9/22 ,  C12N15/63 ,  C12N15/902 ,  C12N15/907 ,  C12P19/34 ,  C12Y301/21004

摘要： Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.