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公开(公告)号：EP4108772A1
公开(公告)日：2022-12-28
申请号：EP21757281.7
申请日：2021-02-19
发明人： EBINUMA Hiroyuki , SUZUKI Ikumi
IPC分类号： C12N15/12 , C12Q1/686 , C12Q1/6876 , C12N9/00
摘要： It is an object of the present disclosure to provide a method for reducing the influence of baseline disturbances in a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR using a fluorescent dye, with a simple method. The present disclosure provides a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR, the method including: (1) amplifying the target nucleic acid in the dry blood filter paper by applying thermal cycles to a sample solution containing a dry blood filter paper punch piece and a PCR reagent, wherein the PCR reagent includes a fluorescently labeled probe; (2) optically detecting the fluorescence intensity of the sample solution for each of the thermal cycles; and (3) performing quantitative analysis of the target nucleic acid using data after a predetermined number of cycles of the optically detected data.
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公开(公告)号：EP4006155A1
公开(公告)日：2022-06-01
申请号：EP20846919.7
申请日：2020-07-22
发明人： EBINUMA Hiroyuki , SUZUKI Ikumi
IPC分类号： C12N15/12 , C12Q1/686 , C12Q1/6876 , C12Q1/6883
摘要： An object of the invention is to provide a primer for detecting and quantifying homozygous deletion of the SMN1 gene, the deletion of which causes SMA, using a dried blood spot in a filter paper and a method related to specific detection/quantification of the SMN1 gene using the primer. The invention is a method for detecting the SMN1 gene in a dried blood spot in a filter paper by real-time PCR, including the steps of (A) to (D) below: (A) a step of adding the dried blood spot in a filter paper to a PCR reaction tube; (B) a step of adding a PCR reagent to the PCR reaction tube, wherein the PCR reagent contains at least a primer designed in a manner that the reactivity to the SMN2 gene is less than 1% of that to the SMN1 gene, a polymerase, dNTPs and an intercalator or a fluorescently labeled probe; (C) a step of performing PCR reaction in the tube containing the PCR reagent and the dried blood spot in a filter paper; and (D) a step of sequentially and optically detecting a target nucleic acid in the SMN1 gene amplified by the PCR reaction.
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公开(公告)号：EP4459281A1
公开(公告)日：2024-11-06
申请号：EP22916103.9
申请日：2022-12-27
发明人： YAMADA Daisuke , SUZUKI Ikumi , FUJIMURA Kengo , ASAI Tomohide , MIYAZAKI Osamu
IPC分类号： G01N33/53 , G01N33/543
摘要： It is an object of the present invention to provide a method for suppressing non-specific reactions using a highly versatile method without affecting specific reactions. The present invention provides a detection method for detecting a target antigen in a sample using a specific antibody that specifically binds to the antigen, the method comprising: a) a step of bringing the sample into contact with a modified antibody; and b) a step of bringing the sample into contact with the specific antibody simultaneously with or after the step a), which is a highly versatile method that can suppress non-specific reactions.
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公开(公告)号：EP4067904A1
公开(公告)日：2022-10-05
申请号：EP20893719.3
申请日：2020-11-27
发明人： ASAI Tomohide , TANAKA Yoshihiro , SUZUKI Ikumi , SEO Satoko , USUI Tomoyuki , SHIMIZU Tomo , MIYAZAKI Osamu
IPC分类号： G01N33/53 , G01N33/543
摘要： A problem to be solved by the invention is to improve the sensitivity of measurement of an analyte in a sample by a sandwich immunoassay. The sensitivity can be improved significantly in a sandwich immunoassay using a first antibody and a second antibody when one or both of the first antibody and the second antibody are a mixture of a monoclonal antibody recognizing a linear epitope and a monoclonal antibody recognizing a conformational epitope.
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