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公开(公告)号：EP4239064A1
公开(公告)日：2023-09-06
申请号：EP23159002.7
申请日：2023-02-28
申请人： Sumitomo Rubber Industries, Ltd. , Tohoku University , Kanazawa University , National University Corporation Saitama University
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi , TOZAWA, Yuzuru
摘要： Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
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公开(公告)号：EP4245851A1
公开(公告)日：2023-09-20
申请号：EP23159026.6
申请日：2023-02-28
申请人： Sumitomo Rubber Industries, Ltd. , Tohoku University , Kanazawa University , National University Corporation Saitama University
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi , TOZAWA, Yuzuru
摘要： Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
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公开(公告)号：EP4239065A1
公开(公告)日：2023-09-06
申请号：EP23159034.0
申请日：2023-02-28
申请人： Sumitomo Rubber Industries, Ltd. , Tohoku University , Kanazawa University , National University Corporation Saitama University
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi , TOZAWA, Yuzuru
摘要： Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles, and mutating the amino acid sequence of a C-terminal region of the cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
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公开(公告)号：EP4050024A1
公开(公告)日：2022-08-31
申请号：EP20891208.9
申请日：2020-10-27
申请人： Sumitomo Rubber Industries, Ltd. , TOHOKU UNIVERSITY , Kanazawa University , National University Corporation Saitama University
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi , TOZAWA, Yuzuru
IPC分类号： C07K14/415 , C07K19/00 , C12P5/02 , C12N5/10 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/63 , C12N9/10
摘要： Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The present invention relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, with the enzymatic activity of the second amino acid sequence being maintained.
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公开(公告)号：EP3992295A1
公开(公告)日：2022-05-04
申请号：EP20841258.5
申请日：2020-06-23
申请人： Sumitomo Rubber Industries, Ltd. , Tohoku University , Kanazawa University , National University Corporation Saitama University
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi , TOZAWA, Yuzuru
IPC分类号： C12N15/62 , C07K14/415 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/29 , C12N15/54 , C12N15/63 , C12P5/00
摘要： Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, the first and second amino acid sequences being fused via a linker sequence (third amino acid sequence) while maintaining the enzymatic activity of the second amino acid sequence.
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公开(公告)号：EP4116423A1
公开(公告)日：2023-01-11
申请号：EP21764148.9
申请日：2021-02-22
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , FUSHIHARA, Kazuhisa , TAKAHASHI, Seiji , NAKAYAMA, Toru , YAMASHITA, Satoshi
摘要： The present invention provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present invention relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.
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7.发明公开PRODUCTION METHOD FOR RUBBER PARTICLES HAVING MEMBRANE-BINDING PROTEIN BOUND THERETO, PRODUCTION METHOD FOR PNEUMATIC TIRE, AND PRODUCTION METHOD FOR RUBBER PRODUCT 审中-公开具有结合了膜结合蛋白质的橡胶粒子的制造方法，气动轮胎的制造方法以及橡胶制品的制造方法
公开(公告)号：EP3305832A1
公开(公告)日：2018-04-11
申请号：EP16817613.9
申请日：2016-05-31
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , FUSHIHARA, Kazuhisa , TAKAHASHI, Seiji , YAMASHITA, Satoshi , NAKAYAMA, Toru , TOZAWA, Yuzuru
CPC分类号： C07K14/415 , B29D30/00 , B29D30/0601 , B29D2030/0055 , B60C1/00 , C08G81/02 , C08H1/00 , C08J2307/02 , C12N15/09 , C12N15/62
摘要： The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
摘要翻译： 本发明提供了通过无细胞蛋白质合成生产与膜结合蛋白质结合的橡胶粒子的方法。 本发明涉及一种生产与膜结合蛋白结合的橡胶粒子的方法，该方法包括在存在橡胶粒子和含有编码膜的mRNA的无细胞蛋白质合成溶液的情况下进行蛋白质合成的步骤 相关的蛋白质将膜相关蛋白质结合到橡胶颗粒上。
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公开(公告)号：EP4324839A1
公开(公告)日：2024-02-21
申请号：EP22787967.3
申请日：2022-03-23
摘要： The present invention aims to provide a method which, even when a protein complex is formed, can purify a protein derived from such a membrane protein complex by eluting the membrane protein complex maintaining its complex form. The present invention relates to a method for purifying a protein derived from a membrane protein complex, which includes: a protein binding step of binding to an antibody a membrane protein complex eluted from a membrane using a synthetic polymer; a protein eluting step of eluting the membrane protein complex bound to the antibody in the protein binding step at a pH of 6.0 or higher; and a synthetic polymer removing step of subjecting the membrane protein complex eluted in the protein eluting step to solvent extraction at a pH of 7.0 or lower to remove the synthetic polymer.
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公开(公告)号：EP3309257B1
公开(公告)日：2020-08-05
申请号：EP16817926.5
申请日：2016-06-28
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10.发明公开PRODUCTION METHOD FOR POLYISOPRENOID, VECTOR, TRANSGENIC PLANT, PRODUCTION METHOD FOR PNEUMATIC TIRE, AND PRODUCTION METHOD FOR RUBBER PRODUCT 审中-公开聚异戊二烯，载体，转基因植物的生产方法，气动轮胎的生产方法以及橡胶产品的生产方法
公开(公告)号：EP3309257A1
公开(公告)日：2018-04-18
申请号：EP16817926.5
申请日：2016-06-28
发明人： YAMAGUCHI, Haruhiko , INOUE, Yukino , FUSHIHARA, Kazuhisa , TAKAHASHI, Seiji , YAMASHITA, Satoshi , NAKAYAMA, Toru
摘要： The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.
摘要翻译： 本发明提供一种生产聚异戊二烯的方法，通过该方法可以提高橡胶颗粒的橡胶合成活性以增加天然橡胶的生产。 本发明涉及体外生产聚类异戊二烯的方法，其涉及使用编码顺式异戊烯转移酶（CPT）家族蛋白的基因和编码Nogo-B受体（NgBR）家族蛋白的基因，并进一步 涉及使用与由这些基因编码的蛋白质结合的橡胶颗粒; 或制备多聚类异戊二烯的方法，其包括向植物中导入载体，其中具有驱动乳管特异性基因表达的启动子活性的启动子与编码CPT家族蛋白的基因和编码NgBR家族的基因连接 蛋白质，以表达laticifers中特异性基因编码的蛋白质。
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