公开(公告)号：EP3733209A1

公开(公告)日：2020-11-04

申请号：EP20164714.6

申请日：2015-10-05

申请人： Synaffix B.V.

发明人： Verkade, Jorge Merijn Mathieu ,  Wijdeven, Maria Antonia ,  van de Sande, Petrus Josephus Jacobus Maria ,  van Berkel, Sander Sebastiaan ,  van Delft, Floris Louis

IPC分类号： A61K47/68 ,  A61P35/00

摘要： The present invention relates to a compound comprising an alpha-end and an omega-end, the compound comprising on the alpha-end a reactive group Q 1 capable of reacting with a functional group F 1 present on a biomolecule and on the omega-end a target molecule, the compound further comprising a group according to formula (1) or a salt thereof:

Said compound may also be referred to as a linker-conjugate. The invention also relates to a process for the preparation of a bioconjugate, the process comprising the step of reacting a reactive group Q 1 of a linker-conjugate according to the invention with a functional group F 1 of a biomolecule. The invention further relates to a bioconjugate obtainable by the process according to the invention.

公开(公告)号：EP4438727A2

公开(公告)日：2024-10-02

申请号：EP24150862.1

申请日：2017-02-08

申请人： Synaffix B.V.

发明人： van Geel, Remon ,  Wijdeven, Maria Antonia ,  Hurkmans, Inge Catharina Josephina ,  van Delft, Floris Louis ,  van Berkel, Sander Sebastiaan

IPC分类号： C12N9/24

CPC分类号： C12Y302/01096 ,  C12N9/24 ,  C07K2319/0020130101 ,  C12N9/2497 ,  C12N15/52 ,  C12N15/62

摘要： The invention concerns fusion proteins, wherein two endoglycosidases are fused, possibly via a linker. The fusion enzymes according to the invention have structure (1): EndoX-(L)p-EndoY (1), wherein EndoX is an endoglycosidase, EndoY is an endoglycosidase distinct from EndoX, L is a linker and p is 0 or 1. Such fusion enzymes capable of trimming glycoproteins comprising at least two distinct glycoforms in a single step. The invention further concerns the use of the fusion enzyme according to the invention for trimming glycoproteins. In another aspect, the invention relates to the process of production of the fusion enzyme. In a further aspect, the inventions concerns a process for trimming glycoproteins, comprising trimming the glycoprotein with a fusion enzyme according to the invention, to obtain a trimmed glycoprotein.

公开(公告)号：EP4148138A1

公开(公告)日：2023-03-15

申请号：EP22185788.1

申请日：2015-08-04

申请人： SynAffix B.V.

发明人： Wasiel, Anna Agnieszka ,  van Delft, Floris Louis ,  van Berkel, Sander Sebastiaan

IPC分类号： C12P21/00 ,  C07H19/10 ,  C07K16/32 ,  C12N9/10

摘要： The present invention relates to a process for the modification of a glycoprotein, using a β-(1,4)-N-acetylgalactosaminyltransferase or a mutant thereof. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of a β-(1,4)-N-acetylgalactosaminyl-transferase or a mutant thereof, with a non-natural sugar-derivative nucleotide. The non-natural sugar-derivative nucleotide is according to formula (3):

wherein A is selected from the group consisting of -N 3 ; -C(O)R 3 ; -C=C-R 4 ; -SH; -SC(O)R 8 ; -SC(V)OR 8 , wherein V is O or S; -X wherein X is selected from the group consisting of F, Cl, Br and I; -OS(O) 2 R 5 ; an optionally substituted C 2 - C 24 alkyl group; an optionally substituted terminal C 2 - C 24 alkenyl group; and an optionally substituted terminal C 3 - C 24 allenyl group.