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    EXPRESSION CASSETTE
    2.
    发明公开
    EXPRESSION CASSETTE 审中-公开
    表达盒

    公开(公告)号：EP2933331A4

    公开(公告)日：2016-08-17

    申请号：EP13862559

    申请日：2013-12-10

    申请人： TAKARA BIO INC

    发明人： DODO KATSUYUKI ,  TSUKIHARA TAKAHISA ,  SHIMOMURA MAYUMI ,  INOUE KOICHI ,  CHONO HIDETO ,  MINENO JUNICHI ,  KITAGAWA MASANARI

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12P21/02

    CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N2740/16311

    摘要： The present invention pertains to: (1) an expression control region which has a sequence corresponding to a transcriptional activator binding domain; and (2) a nucleic acid which codes a transcriptional activator capable of binding to the expression control region. Provided are: an expression cassette including the nucleic acid connected to the expression control region in a manner so as to be capable of functioning; a method for expression of a product of interest in mammalian cells, said method using the expression cassette; a method for production of cells which express the product of interest; a method for production of the product of interest in mammalian cells; and a kit which includes the expression cassette.

    METHOD FOR QUANTIFYING ADENO-ASSOCIATED VIRUS
    4.
    发明公开
    METHOD FOR QUANTIFYING ADENO-ASSOCIATED VIRUS 审中-公开
    VERFAHREN ZUR QUANTIFIZIERUNG VON ADENO-ASSOZIIERTEM VIRUS

    公开(公告)号：EP3075849A4

    公开(公告)日：2017-06-14

    申请号：EP14866486

    申请日：2014-11-27

    申请人： TAKARA BIO INC

    发明人： AKASHIKA TAMAKI ,  DODO KATSUYUKI ,  KITAGAWA MASANARI

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q2600/158

    摘要： A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.

    摘要翻译： 一种定量腺伴随病毒基因组的方法，包括以下步骤：（a）制备含有样品的组合物，至少一个引物对用于扩增仅含有腺相关反向末端重复序列中的核苷酸序列 病毒和嵌入染料; （b）使用步骤（a）中制备的组合物进行核酸扩增反应; 和（c）检测步骤（b）中获得的扩增产物。 本发明在医学，基因工程和生物学领域特别有用。