公开(公告)号：EP1072678A4

公开(公告)日：2006-09-27

申请号：EP99917081

申请日：1999-04-21

申请人： TAKARA BIO INC

发明人： ASADA KIYOZO ,  UEMORI TAKASHI ,  SATO YOSHIMI ,  FUJITA TOMOKO ,  MIYAKE KAZUE ,  TAKEDA OSAMU ,  MUKAI HIROYUKI ,  KATO IKUNOSHIN

IPC分类号： C12N9/12 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6806 ,  Y10S435/975 ,  C12Q2521/107

摘要： DNA synthesizing reaction promoters containing at least one member selected from the group consisting of acidic substances and cation complexes; a method for synthesizing DNA wherein a DNA synthesizing reaction is effected by using a DNA polymerase in the presence of the above promoters; compositions for DNA synthesizing reactions which contain the above promoters; compositions for DNA synthesizing reactions which contain at least two DNA polymerases having 3'→5' exonuclease activity; a method for synthesizing DNA wherein a DNA synthesizing reaction is effected by using at least two DNA polymerases having 3'→5' exonuclease activity; kits to be used in synthesizing DNA in vitro which contain at least two DNA polymerases having 3'→5' exonuclease activity; and kits to be used in synthesizing DNA in vitro which contain the above-mentioned promoters and DNA polymerases. Thus, DNA can be synthesized at a higher efficiency than in the conventional DNA synthesizing reactions.

公开(公告)号：EP1167524A4

公开(公告)日：2005-03-09

申请号：EP00908056

申请日：2000-03-14

申请人： TAKARA BIO INC

发明人： MUKAI HIROYUKI ,  YAMAMOTO JUNKO ,  TAKEDA OSAMU ,  MIYAKE KAZUE ,  UEMORI TAKASHI ,  SATO YOSHIMI ,  MORIYAMA MARIKO ,  SAWARAGI HARUHISA ,  HAGIYA MICHIO ,  ASADA KIYOZO ,  KATO IKUNOSHIN

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N15/11

CPC分类号： C12Q1/6844 ,  C12Q2531/119 ,  C12Q2525/121 ,  C12Q2521/319

摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

公开(公告)号：EP0997530A4

公开(公告)日：2004-12-08

申请号：EP98929687

申请日：1998-06-24

申请人： TAKARA BIO INC

发明人： UEMORI TAKASHI ,  SATO YOSHIMI ,  FUJITA TOMOKO ,  MIYAKE KAZUE ,  MUKAI HIROYUKI ,  ASADA KIYOZO ,  KATO IKUNOSHIN

IPC分类号： C12N9/12 ,  C12N15/54 ,  C07K14/195 ,  C12Q1/68

CPC分类号： C12N9/1252

摘要： A thermostable DNA polymerase-related factor promoting the DNA synthesizing activities of DNA polymerases; a thermostable DNA polymerase-related factor having the activity of binding to DNA polymerases and a process for producing the same; genes encoding the above DNA polymerase-related factors; a method for synthesizing DNAs wherein DNA polymerases are used in the presence of the above DNA polymerase-related factors; and kits containing the above DNA polymerase-related factors. Use of these DNA polymerase-related factors makes it possible to provide DNA synthesis systems and DNA amplification systems in vitro which are superior to the conventional ones.