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公开(公告)号：EP0170345A2
公开(公告)日：1986-02-05
申请号：EP85302094.9
申请日：1985-03-26
CPC分类号： G01N33/56972
摘要： A flow cytometry method reproducibly detects and counts a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color- producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.
摘要翻译： 流式细胞术方法可重复地检测并计数随后裂解红细胞的白细胞悬浮液或全血样品中感兴趣的淋巴细胞群。 将悬浮液（或样品）与包含天然的，携带附着的酶或生物素或其它标记的一级抗体和固定剂的试剂以任何顺序组合。 当酶不连接时，酶与第一抗体特异性地偶联。 将固定的悬浮液与产生色素的酶 - 细胞化学试剂反应。 将包含染色和未染色的固定细胞的悬浮液通过流式细胞仪，并根据其光散射和光吸收特性对细胞进行表征和计数。
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公开(公告)号：EP0170345A3
公开(公告)日：1987-03-25
申请号：EP85302094
申请日：1985-03-26
申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation) , THE MOUNT SINAI SCHOOL OF MEDICINE
CPC分类号： G01N33/56972
摘要： A flow cytometry method reproducibly detects and counts a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color- producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.
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4.发明公开Method and apparatus for separating blood lymphocytes from blood 失效Verfahren und Vorrichtung zum Abtrennen von Blutlymphozyten aus Blut。
公开(公告)号：EP0119692A2
公开(公告)日：1984-09-26
申请号：EP84300435.9
申请日：1984-01-25
发明人： Kanter, Robert J.
CPC分类号： G01N33/491 , B01D21/262 , B01D43/00 , B01D2221/10 , Y10T436/25375
摘要： In an apparatus and method for separating, by centrifugation, blood lymphocytes from other leukocytes in a blood sample, small dense particles (7), either wetted or suspended in a physiological, hydrophilic medium, and a chemically inert hydrophobic barrier material (5) are initially located in a suitable container (1). When the blood sample is added to the container, the particles are phagocytized by the monocytes and granulocytes, so as to increase their specific gravities. Upon centrifugation, the barrier material, whose specific gravity is only slightly greater than the specific gravity of the lymphocytes and less than the respective specific gravities of the phagocytic cells which have ingested particles and of the erythrocytes (red blood cells) in the blood sample, forms a barrier therebetween, allowing harvesting of the lymphocytes, substantially free of contamination.
摘要翻译： 在通过离心分离血液样品中来自其他白细胞的血液淋巴细胞的装置和方法中，湿润或悬浮在生理亲水介质和化学惰性疏水屏障材料（5）中的小致密颗粒（7） 最初位于合适的容器（1）中。 当将血液样品加入到容器中时，颗粒被单核细胞和粒细胞吞噬，以增加它们的比重。 离心时，其比重仅比淋巴细胞的比重略高于具有摄取颗粒的吞噬细胞和血液样品中的红细胞（红细胞）的比重的阻隔材料， 在其间形成屏障，允许收集淋巴细胞，基本上没有污染。
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公开(公告)号：EP0119692B1
公开(公告)日：1990-10-03
申请号：EP84300435.9
申请日：1984-01-25
发明人： Kanter, Robert J.
CPC分类号： G01N33/491 , B01D21/262 , B01D43/00 , B01D2221/10 , Y10T436/25375
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