公开(公告)号：EP1153604A1

公开(公告)日：2001-11-14

申请号：EP99903920.9

申请日：1999-02-15

申请人： THE NIKKA WHISKY DISTILLING CO., LTD. ,  Noda, Masatoshi

发明人： NODA, Masatoshi ,  KANDA, Tomomasa ,  YANAGIDA, Akio ,  HIEDA, Kazuo

IPC分类号： A61K31/35 ,  A61K31/78

CPC分类号： A61K31/78 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/35 ,  A61K31/352 ,  A61K31/353 ,  A61K36/73 ,  A61K36/87 ,  Y02A50/469 ,  Y02A50/471 ,  A23V2250/2104

摘要： A composition for the treatment and/or prevention of enterotoxin type bacterial infectious disease comprising, as an active ingredient, a substance containing proanthocyanidin derived from a naturally occurring substance such as an apple or grape extract, which can inhibit toxins produced by enteric infection causative bacteria of enterotoxin type, e.g., pathogenic vibrios (Vibrio cholerae, Vibrio parahaemolyticus); and a composition for the treatment and/or prevention of diphtheria, etc. based on the ADP-ribosylation inhibiting activity of proanthocyanidin; as well as a food additive and foodstuff containing proanthocyanidin or the composition used for the treatment and prevention of these infectious diseases.

摘要翻译： 对于肠毒素型细菌感染性疾病的治疗和/或预防，其包含作为活性成分的组合物，从天然存在的物质衍生的物质，含有花色素原：如苹果或葡萄提取物，它可以抑制由肠道感染的致病细菌产生的毒素 肠毒素类型，例如，致病性弧菌（霍乱弧菌，副溶血性弧菌）; 以及用于基于所述治疗和/或预防白喉等的组合物中的ADP-核糖基化抑制原花色素的活性; 以及作为食品添加剂和食品材料含原花色素，或用于治疗和预防感染性疾病本文的组合物中。

公开(公告)号：EP1180516B1

公开(公告)日：2006-08-23

申请号：EP00906694.5

申请日：2000-03-03

申请人： KYOWA HAKKO KOGYO CO., LTD. ,  THE NIKKA WHISKY DISTILLING CO., LTD.

发明人： HONDA, Shinkichi, H.O. Kyowa Hakko Kogyo Co., Ltd. ,  TAKAHASHI, Tomoya, c/oTsukuba Research Lab. ,  KAMIMURA, Ayako, c/o Tsukuba Research Lab. ,  MATSUOKA, Takako ,  KANDA, Tomomasa ,  YANAGIDA, Akio ,  HIEDA, Kazuo

IPC分类号： C07D311/62

CPC分类号： C07D311/62 ,  C07D311/60

摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.

公开(公告)号：EP1180516A1

公开(公告)日：2002-02-20

申请号：EP00906694.5

申请日：2000-03-03

申请人： KYOWA HAKKO KOGYO CO., LTD. ,  THE NIKKA WHISKY DISTILLING CO., LTD.

发明人： HONDA, Shinkichi, H.O. Kyowa Hakko Kogyo Co., Ltd. ,  TAKAHASHI, Tomoya, Tsukuba Research Laboratories ,  KAMIMURA, Ayako, Tsukuba Research Laboratories ,  MATSUOKA, Takako ,  KANDA, Tomomasa ,  YANAGIDA, Akio ,  HIEDA, Kazuo

IPC分类号： C07D311/62

CPC分类号： C07D311/62 ,  C07D311/60

摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.

摘要翻译： 本发明涉及一种纯化二聚至五聚原花青素低聚物的方法，其中包括通过使用乙酸甲酯作为液相的固液萃取从原花青素低聚物或粗提纯产物中提取原花青素低聚物; 用于纯化二聚体至五聚原花青素低聚物的方法，其包括用酶预处理含有原花青素低聚物的水解原料，其粗提纯产物或含有这些之一的溶液; 以及具有均匀聚合度的二聚至五聚原花青素低聚物的纯化方法，该方法包括通过使用作为流动相的正相硅胶液相色谱法从含有原花青素低聚物的原料或其纯化产物中分离纯化原花青素低聚物 单一溶剂或选自酯溶剂，酮溶剂，烃溶剂，醚溶剂和醇溶剂中的两种或更多种溶剂的混合溶剂。