专利检索 ap:("THE UNIVERSITY OF WYOMING") AND inv:"HALEY, Boyd, E. Markey Cancer Center" 第 1 页

1.

发明授权
5-AZIDO DEOXYURIDINE COMPOUNDS AND METHOD OF SYNTHESIS OF THE SAME 失效
标题翻译： 5-氨基脱氧羟基化合物及其合成方法

公开(公告)号：EP0218701B1

公开(公告)日：1993-08-04

申请号：EP86902752.4

申请日：1986-04-22

申请人： THE UNIVERSITY OF WYOMING

发明人： HALEY, Boyd, E. Markey Cancer Center , EVANS, Robert, K. Markey Cancer Center

IPC分类号： C07H19/06 , C07H19/067 , C07H19/073 , C07H19/10

CPC分类号： C07H19/10

摘要： 5-azido-2'-deoxyuridine nucleoside and nucleotide compounds and a method of producing them. The method involves reacting a 5'-deoxyuridine compound with nitrosonium tetrafluoroborate to produce a 5-nitro-deoxyuridine compound. The 5-nitro-deoxyuridine compound is reduced to a 5-amino-deoxyuridine compound in the presence of metallic zinc. The 5-amino-deoxyuridine compound is acidified and reacted with sodium nitrite to produce a 5-diazo-deoxyuridine compound. The diazo-deoxyuridine compound is then reacted with sodium azide to produce a 5-azido-deoxyuridine compound. The latter, in the monophosphate form, is further reacted with diphenylchlorophosphate to produce the photoactive triphosphate, 5-azido-2'-deoxyuridine-5'-triphosphate or 5-azido-dUTP. The photoactive compound, when added to DNA in an in vitro synthesis reaction, produces photoactive DNA.