公开(公告)号：EP1586639A4

公开(公告)日：2007-09-19

申请号：EP02760765

申请日：2002-08-28

Applicant: TOSHIBA KK

Inventor： TAKAHASHI MASAYOSHI ,  OKADA JUN ,  HASHIMOTO KOJI

IPC: C12Q1/68 ,  C12N15/09 ,  G01N33/50

CPC classification number: C12Q1/6837 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2563/107 ,  C12Q2563/113

Abstract: A nucleic acid probe immobilization support comprising a support and a nucleic acid probe which has been immobilized on the support via a spacer and contains a base sequence complementary to a target sequence, characterized by fulfilling the relationship X ≥ Y; wherein X stands for the length of the spacer; and Y stands for the distance from the end of the hybridization site in the support side to the end of a target nucleic acid in the support side in case of hybridizing the nucleic acid probe with the target nucleic acid having the target sequence as a part thereof.

公开(公告)号：EP2740788A4

公开(公告)日：2015-08-19

申请号：EP12822401

申请日：2012-08-03

Applicant: TOSHIBA KK

Inventor： TAKAHASHI MASAYOSHI ,  HASHIMOTO KOJI ,  NIKAIDO MASARU ,  GEMMA NOBUHIRO ,  OKADA JUN ,  HIROSAWA DAIJI ,  YAMAMOTO KEIICHI ,  KUWABARA TETSUYA ,  TAKASE MADOKA ,  SHIMOJO AKIKO ,  NAKAMURA AKIYOSHI

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N27/327

CPC classification number: C12Q1/6825 ,  B01J2219/00452 ,  B01J2219/00497 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L7/525 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/163

公开(公告)号：EP1530043A4

公开(公告)日：2008-03-19

申请号：EP04714496

申请日：2004-02-25

Applicant: TOSHIBA KK

Inventor： O'UCHI SHIN-ICHI ,  FUNAKI HIDEYUKI ,  HONGO SADATO ,  HASHIMOTO KOJI ,  GEMMA NOBUHIRO ,  OKADA JUN ,  TAKAHASHI MASAYOSHI

IPC: G01N33/53 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N27/327 ,  G01N27/416 ,  G01N27/49 ,  G01N33/566 ,  G01N37/00

CPC classification number: C12M1/34 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2200/143 ,  B01L2200/148 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/087 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6837 ,  C40B40/06

Abstract: The present invention includes a plurality of working electrodes (141) on which the same type of nucleic acid probes each having a nucleic acid complementary to a target nucleic acid are immobilized and which have different sensor areas and a normalization circuit which normalizes detection signals obtained by the working electrodes (141) with respect to the respective sensor areas.

公开(公告)号：EP1445609A4

公开(公告)日：2005-04-06

申请号：EP02762891

申请日：2002-08-28

Applicant: TOSHIBA KK

Inventor： OKADA JUN ,  HASHIMOTO KOJI ,  TAKAHASHI MASAYOSHI

IPC: G01N33/483 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/327 ,  G01N27/416 ,  G01N33/53 ,  G01N37/00 ,  G01N27/30

CPC classification number: C12Q1/6825 ,  C12Q2563/173 ,  C12Q2549/125

Abstract: A base sequencing device comprising a conductive sensing electrode (2), blocking molecules (21) which are formed to cover the surface of the sensing electrode (2) to reduce adsorption of an inserting agent by the surface of the sensing electrode (2), a target complementary nucleic acid probe (23) which is immobilized on the sensing electrode (2) via a spacer member (22) formed of normal chain organic molecules and has a basic sequence complementary to a target basic sequence to be determined, a conductive reference electrode (3), and blocking molecules (31) which are formed to cover the surface of the reference electrode (3) to reduce adsorption of the inserting agent by the surface of the reference electrode (3).