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    METHOD FOR PRODUCING NON-ENVELOPED VIRAL PARTICLES
    3.
    发明公开
    METHOD FOR PRODUCING NON-ENVELOPED VIRAL PARTICLES 审中-公开
    生产非包装病毒颗粒的方法

    公开(公告)号：EP3243904A1

    公开(公告)日：2017-11-15

    申请号：EP16735082.6

    申请日：2016-01-08

    申请人： Takara Bio Inc.

    发明人： KAWANO, Yasuhiro ,  HUANG, Shuohao ,  ENOKI, Tatsuji ,  KITAGAWA, Masanari

    IPC分类号： C12N7/02 ,  C07K14/075 ,  C12N5/10 ,  C12N15/09

    CPC分类号： C07K14/075 ,  C12N5/10 ,  C12N7/02 ,  C12N15/09

    摘要： Provided is a method for producing non-enveloped viral particles, comprising a step for obtaining a fraction containing non-enveloped viral particles by removing precipitates which are produced in a step for adding a substance which reduces the solubility of proteins under acidic conditions and/or a substance which precipitates under acidic conditions to a neutral or basic sample containing non-enveloped viral particles, and acidifying the sample after the addition of the substance.

    摘要翻译： 提供一种生产无包膜病毒颗粒的方法，该方法包括通过除去在酸性条件下降低蛋白质溶解度的物质的步骤中产生的沉淀物和/或 在酸性条件下沉淀成含有无包膜病毒颗粒的中性或碱性样品，并在加入该物质后酸化样品的物质。

    METHOD FOR QUANTIFYING ADENO-ASSOCIATED VIRUS
    5.
    发明公开
    METHOD FOR QUANTIFYING ADENO-ASSOCIATED VIRUS 审中-公开
    方法定量腺相关病毒

    公开(公告)号：EP3075849A1

    公开(公告)日：2016-10-05

    申请号：EP14866486.5

    申请日：2014-11-27

    申请人： Takara Bio, Inc.

    发明人： AKASHIKA, Tamaki ,  DODO, Katsuyuki ,  KITAGAWA, Masanari

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q2600/158

    摘要： A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.

    EXPRESSION CASSETTE
    7.
    发明公开
    EXPRESSION CASSETTE 审中-公开
    EXPRESSIONSKASSETTE

    公开(公告)号：EP2933331A1

    公开(公告)日：2015-10-21

    申请号：EP13862559.5

    申请日：2013-12-10

    申请人： Takara Bio, Inc.

    发明人： DODO, Katsuyuki ,  TSUKIHARA, Takahisa ,  SHIMOMURA, Mayumi ,  INOUE, Koichi ,  CHONO, Hideto ,  MINENO, Junichi ,  KITAGAWA, Masanari

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12P21/02

    CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N2740/16311

    摘要： The present invention pertains to: (1) an expression control region which has a sequence corresponding to a transcriptional activator binding domain; and (2) a nucleic acid which codes a transcriptional activator capable of binding to the expression control region. Provided are: an expression cassette including the nucleic acid connected to the expression control region in a manner so as to be capable of functioning; a method for expression of a product of interest in mammalian cells, said method using the expression cassette; a method for production of cells which express the product of interest; a method for production of the product of interest in mammalian cells; and a kit which includes the expression cassette.

    摘要翻译： 本发明涉及：（1）具有对应于转录激活子结合结构域的序列的表达控制区; 和（2）编码能够结合表达控制区的转录激活子的核酸。 提供：包括以能够起作用的方式连接到表达控制区的核酸的表达盒; 用于在哺乳动物细胞中表达感兴趣的产物的方法，所述使用表达盒的方法; 用于生产表达感兴趣的产物的细胞的方法; 生产哺乳动物细胞感兴趣的产物的方法; 以及包括表达盒的试剂盒。