公开(公告)号：EP3083975A1

公开(公告)日：2016-10-26

申请号：EP14816252.2

申请日：2014-12-19

申请人： Technical University of Denmark ,  University of Copenhagen ,  The University of British Columbia

发明人： HAMBERGER, Björn ,  PATERAKI, Eirini ,  MØLLER, Birger Lindberg ,  NØRHOLM, Morten ,  NIELSEN, Morten Thrane ,  ANDERSEN-RANBERG, Johan ,  BOHLMANN, Carl Jörg ,  ZERBE, Philipp

IPC分类号： C12P17/02 ,  C07D311/00 ,  C12N9/16 ,  C12N15/52

CPC分类号： C07D311/92 ,  C07B2200/07 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8243 ,  C12P17/06 ,  C12Y402/03 ,  C12Y505/01

摘要： The present invention relates to a method for manufacturing enantiomerically pure (13R)-manoyl oxide, said method comprising the steps of contacting geranylgeranyl diphosphate (GGPP) with a class II diterpene synthase to obtain labd-13-en-8,15-diol diphosphate (LPP), and then contacting the LPP with a class I diterpene synthase to obtain (13R)-manoyl oxide. The invention further relates to (13R)-manoyl oxide obtained by the method of the invention.

摘要翻译： 本发明涉及一种制备对映异构纯的（13R） - 马来酰氧基氧化物的方法，所述方法包括以下步骤：将香叶基香叶基二磷酸酯（GGPP）与二级二萜合成酶接触，得到labd-13-en-8,15-二醇二磷酸酯 （LPP），然后使LPP与I类二萜合酶接触，得到（13R） - 马来酰氧基。 本发明还涉及通过本发明的方法获得的（13R） - 马来酰氧基氧化物。

公开(公告)号：EP3083975B1

公开(公告)日：2018-11-14

申请号：EP14816252.2

申请日：2014-12-19

申请人： Technical University of Denmark ,  University of Copenhagen

发明人： HAMBERGER, Björn ,  PATERAKI, Eirini ,  MØLLER, Birger Lindberg ,  NØRHOLM, Morten ,  NIELSEN, Morten Thrane ,  ANDERSEN-RANBERG, Johan ,  BOHLMANN, Carl Jörg ,  ZERBE, Philipp

IPC分类号： C12P17/02 ,  C07D311/00 ,  C12N9/16 ,  C12N15/52

CPC分类号： C07D311/92 ,  C07B2200/07 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8243 ,  C12P17/06 ,  C12Y402/03 ,  C12Y505/01

摘要： The present invention relates to a method for manufacturing enantiomerically pure (13R)-manoyl oxide, said method comprising the steps of contacting geranylgeranyl diphosphate (GGPP) with a class II diterpene synthase to obtain labd-13-en-8,15-diol diphosphate (LPP), and then contacting the LPP with a class I diterpene synthase to obtain (13R)-manoyl oxide. The invention further relates to (13R)-manoyl oxide obtained by the method of the invention.

公开(公告)号：EP3215626A1

公开(公告)日：2017-09-13

申请号：EP15804062.6

申请日：2015-11-05

申请人： University of Copenhagen

发明人： ANDERSEN-RANBERG, Johan ,  PATERAKI, Eirini

IPC分类号： C12P17/06

CPC分类号： C12P17/06

摘要： The invention discloses novel methods for biosynthesis of forskolin and other oxidised 13R-MOs. Oxidised 13R-MO may be valuable on its own account or as precursors for production of forskolin. In particular, the invention provides methods of producing an oxidised 13R-manoyl oxide (13R-MO) comprising the steps of providing a host organism comprising a heterologous nucleic acid encoding an enzyme capable of catalysing hydroxylation of 13R-MO and/or oxidised 13R-MO at the 9 position, wherein said oxidised 13R-MO carries a –H at the 9-position and incubating said host organism in the presence of 13R-MO under conditions allowing growth of said host organism. The invention also discloses materials for use in said methods, in particular the invention provides the enzyme CYP76AH16.

摘要翻译： 本发明公开了用于福斯高林和其他氧化的13R-MO的生物合成的新方法。 氧化的13R-MO本身可能是有价值的，或作为用于生产毛喉素的前体。 具体而言，本发明提供了生产氧化的13R-甘露糖基氧化物（13R-MO）的方法，包括以下步骤：提供包含编码能够催化13R-MO和/或氧化的13R-MO的羟基化的酶的异源核酸的宿主生物体， 其中所述氧化的13R-MO在9位携带-H，并在存在13R-MO的情况下在允许所述宿主生物生长的条件下孵育所述宿主生物体。 本发明还公开了用于所述方法的材料，特别是本发明提供了酶CYP76AH16。

公开(公告)号：EP3099802A1

公开(公告)日：2016-12-07

申请号：EP15706364.5

申请日：2015-01-30

申请人： The University of Copenhagen ,  Danmarks Tekniske Universitet ,  Evolva SA

发明人： HAMBERGER, Björn ,  MØLLER, Birger Lindberg ,  PATERAKI, Eirini ,  ANDERSEN-RANBERG, Johan ,  JENSEN, Niels Bjerg

IPC分类号： C12P5/00 ,  C12P17/06 ,  C12N9/02

CPC分类号： C12P17/06 ,  C12N9/0071 ,  C12P5/007 ,  C12Y114/14001

摘要： The invention described materials and methods for producing oxidised 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R- MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.

公开(公告)号：EP3283623A1

公开(公告)日：2018-02-21

申请号：EP16717341.8

申请日：2016-04-14

申请人： Evolva SA ,  University of Copenhagen

发明人： HAMBERGER, Björn ,  ANDERSEN-RANBERG, Johan ,  JENSEN, Niels Bjerg ,  PATERAKI, Eirini ,  MØLLER, Birger Lindberg

IPC分类号： C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12P5/00

CPC分类号： C12P17/06 ,  C12N9/1029 ,  C12P5/007

摘要： The invention relates to recombinant microorganisms and methods for producing acetylated diterpenes, including oxidized and/or acetylated oxidized diterpenes such as forskolin.