公开(公告)号：EP3083975A1

公开(公告)日：2016-10-26

申请号：EP14816252.2

申请日：2014-12-19

申请人： Technical University of Denmark ,  University of Copenhagen ,  The University of British Columbia

发明人： HAMBERGER, Björn ,  PATERAKI, Eirini ,  MØLLER, Birger Lindberg ,  NØRHOLM, Morten ,  NIELSEN, Morten Thrane ,  ANDERSEN-RANBERG, Johan ,  BOHLMANN, Carl Jörg ,  ZERBE, Philipp

IPC分类号： C12P17/02 ,  C07D311/00 ,  C12N9/16 ,  C12N15/52

CPC分类号： C07D311/92 ,  C07B2200/07 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8243 ,  C12P17/06 ,  C12Y402/03 ,  C12Y505/01

摘要： The present invention relates to a method for manufacturing enantiomerically pure (13R)-manoyl oxide, said method comprising the steps of contacting geranylgeranyl diphosphate (GGPP) with a class II diterpene synthase to obtain labd-13-en-8,15-diol diphosphate (LPP), and then contacting the LPP with a class I diterpene synthase to obtain (13R)-manoyl oxide. The invention further relates to (13R)-manoyl oxide obtained by the method of the invention.

摘要翻译： 本发明涉及一种制备对映异构纯的（13R） - 马来酰氧基氧化物的方法，所述方法包括以下步骤：将香叶基香叶基二磷酸酯（GGPP）与二级二萜合成酶接触，得到labd-13-en-8,15-二醇二磷酸酯 （LPP），然后使LPP与I类二萜合酶接触，得到（13R） - 马来酰氧基。 本发明还涉及通过本发明的方法获得的（13R） - 马来酰氧基氧化物。

公开(公告)号：EP3083975B1

公开(公告)日：2018-11-14

申请号：EP14816252.2

申请日：2014-12-19

申请人： Technical University of Denmark ,  University of Copenhagen

发明人： HAMBERGER, Björn ,  PATERAKI, Eirini ,  MØLLER, Birger Lindberg ,  NØRHOLM, Morten ,  NIELSEN, Morten Thrane ,  ANDERSEN-RANBERG, Johan ,  BOHLMANN, Carl Jörg ,  ZERBE, Philipp

IPC分类号： C12P17/02 ,  C07D311/00 ,  C12N9/16 ,  C12N15/52

CPC分类号： C07D311/92 ,  C07B2200/07 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8243 ,  C12P17/06 ,  C12Y402/03 ,  C12Y505/01

摘要： The present invention relates to a method for manufacturing enantiomerically pure (13R)-manoyl oxide, said method comprising the steps of contacting geranylgeranyl diphosphate (GGPP) with a class II diterpene synthase to obtain labd-13-en-8,15-diol diphosphate (LPP), and then contacting the LPP with a class I diterpene synthase to obtain (13R)-manoyl oxide. The invention further relates to (13R)-manoyl oxide obtained by the method of the invention.

公开(公告)号：EP3099803A1

公开(公告)日：2016-12-07

申请号：EP15706365.2

申请日：2015-01-30

申请人： University of Copenhagen ,  Danmarks Tekniske Universitet

发明人： HAMBERGER, Björn ,  MØLLER, Birger Lindberg ,  ANDERSEN-RANBERG, Johan ,  BOHLMANN, Carl Jörg ,  ZERBE, Philipp ,  NIELSEN, Morten Thrane

IPC分类号： C12P5/00 ,  C12P7/00 ,  C07K14/415 ,  C12N9/00

CPC分类号： C12P5/007 ,  C07K14/415 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/88 ,  C12P7/00 ,  C12P17/06 ,  C12Y204/01015 ,  C12Y301/03012

摘要： The present invention discloses that by combining different di TPS enzymes of class I and class II different diterpenes may be produced including diterpenes not identified in nature. Surprisingly it is revealed that a di TPS enzyme of class I of one species may be combined with a di TPS enzyme of class II from a different species, resulting in a high diversity of diterpenes, which can be produced.

摘要翻译： 本发明公开了通过组合I类和II类不同的二TPS酶，可以制备不同的二萜类，包括本质上未鉴定的二萜烯。 令人惊奇地发现，一种物质的I类的二TPS酶可以与来自不同物种的II类的二TPS酶组合，导致可以产生高的多萜类化合物。