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    PEPTIDE PURIFICATION USING MIXED-PHASE SOLID PHASE EXTRACTION MATERIAL
    5.
    发明公开
    PEPTIDE PURIFICATION USING MIXED-PHASE SOLID PHASE EXTRACTION MATERIAL 审中-公开
    用混合相固相萃取材料进行肽类的纯化

    公开(公告)号：EP3319977A1

    公开(公告)日：2018-05-16

    申请号：EP16738402.3

    申请日：2016-07-08

    申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

    发明人： KULAK, Nils A. ,  PICHLER, Garwin ,  MANN, Matthias

    IPC分类号： C07K1/16

    CPC分类号： C07K1/165 ,  G01N33/5002

    摘要： The present invention relates to a method of purifying peptides and/or polypeptides, said method comprising or consisting of: (a) loading a sample comprising peptides and/or polypeptides under acidic or neutral aqueous conditions on mixed-phase solid phase extraction (SPE) material, wherein said material consists of or comprises reversed phase/ion exchange material; (b) washing said mixed-phase SPE material with (ba) an acidic or neutral composition comprising at least 50% (vlv) organic solvent; and/or (bb) an acidic or neutral aqueous solution; and (c) eluting said peptides and/or polypeptides from said mixed-phase SPE material with an alkaline composition comprising at least 50% (v/v) organic solvent.

    REACTION VESSEL FOR SAMPLE PREPARATION
    6.
    发明公开
    REACTION VESSEL FOR SAMPLE PREPARATION 审中-公开
    REAKTIONSGEFÄSSZUR PROBENVORBEREITUNG

    公开(公告)号：EP2934749A2

    公开(公告)日：2015-10-28

    申请号：EP13815477.8

    申请日：2013-12-19

    申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

    发明人： KULAK, Nils A. ,  MANN, Matthias ,  AZIMIFAR, Seyed Babak ,  NAGARAJ, Nagarjuna

    IPC分类号： B01L3/00 ,  B01D15/22 ,  G01N30/02 ,  G01N30/60

    CPC分类号： G01N1/405 ,  B01D15/12 ,  B01D15/125 ,  B01D15/22 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/069 ,  C12N15/101 ,  G01N33/6842

    摘要： The present invention relates to sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, the container comprising a reaction chamber and a chromatography medium; wherein said reaction chamber is for holding said cellular material, viruses and/or sub-components of said cellular material and/or viruses and is configured such that at least one of the following reactions can be performed therein: lysis, e.g. by sonication and/or boiling; chromatographic purification; reduction; alkylation; and enzymatic reactions such as proteolysis; wherein said chromatography medium is configured to purify and/or enrich said bio-organic compounds; wherein (a) said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds; or (b) said sample preparation container further comprises a receiving chamber for receiving said bio-organic compounds, said receiving chamber being adjacent to said chromatography medium such that said chromatography medium separates said reaction chamber from said receiving chamber, and the outer face of said receiving chamber is closed and configured to be opened for obtaining purified and/or enriched bio-organic compounds.

    摘要翻译： 本发明涉及用于从所述细胞材料和/或病毒的细胞材料，病毒和/或亚组分纯化和/或富集生物有机化合物的样品制备容器，所述容器包含反应室和色谱介质; 其中所述反应室用于保持所述细胞材料，所述细胞材料和/或病毒的病毒和/或亚组分，并且被配置为使得可以在其中进行至少一个以下反应： 通过超声处理和/或煮沸; 色谱纯化; 减少; 烷基化; 和酶反应如蛋白水解; 其中所述色谱介质被配置为净化和/或富集所述生物有机化合物; 其中（a）所述色谱介质位于所述反应室的壁处，并且所述壁被封闭或密封并构造成打开以获得纯化和/或富集的生物有机化合物; 或（b）所述样品制备容器还包括用于接收所述生物有机化合物的接收室，所述接收室与所述色谱介质相邻，使得所述色谱介质将所述反应室与所述接收室分离，并且所述 接收室被关闭并被构造成打开以获得纯化和/或富集的生物有机化合物。