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公开(公告)号:EP4291630A1
公开(公告)日:2023-12-20
申请号:EP22707558.7
申请日:2022-02-11
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公开(公告)号:EP3254008A1
公开(公告)日:2017-12-13
申请号:EP16708248.6
申请日:2016-02-04
IPC分类号: F16K99/00
CPC分类号: B01L3/502738 , B01L2200/027 , B01L2300/0816 , B01L2300/12 , B01L2300/123 , B01L2300/14 , B01L2400/0638 , B01L2400/082 , C12M21/08 , C12M23/16 , F16K7/126 , F16K99/0015 , F16K2099/008
摘要: Systems and methods disclosed herein related to an apparatus including a fluid flow plate and a microfluidic valve assembly. The fluid flow plate includes a plurality of polymer layers that define a fluid flow passage through the microfluidic valve assembly. The microfluidic valve assembly includes a valve seat, a flexible membrane, a valve cavity, a valve head, and an actuator. The actuator is configured to selectively control pressure applied by the valve head to the flexible membrane, such that in a first actuator state the valve head depresses the flexible membrane into the valve cavity and into contact with the valve seat, thereby preventing fluid flow through the valve assembly, and in a second state, the valve head and the flexible membrane are retracted substantially out of the valve cavity allowing fluid to flow through the valve assembly. In various implementations, the valve seat and/or the flexible membrane include an elastomer layer.
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公开(公告)号:EP3254008B1
公开(公告)日:2018-12-26
申请号:EP16708248.6
申请日:2016-02-04
IPC分类号: F16K99/00
CPC分类号: B01L3/502738 , B01L2200/027 , B01L2300/0816 , B01L2300/12 , B01L2300/123 , B01L2300/14 , B01L2400/0638 , B01L2400/082 , C12M21/08 , C12M23/16 , F16K7/126 , F16K99/0015 , F16K2099/008
摘要: Systems and methods disclosed herein related to an apparatus including a fluid flow plate and a microfluidic valve assembly. The fluid flow plate includes a plurality of polymer layers that define a fluid flow passage through the microfluidic valve assembly. The microfluidic valve assembly includes a valve seat, a flexible membrane, a valve cavity, a valve head, and an actuator. The actuator is configured to selectively control pressure applied by the valve head to the flexible membrane, such that in a first actuator state the valve head depresses the flexible membrane into the valve cavity and into contact with the valve seat, thereby preventing fluid flow through the valve assembly, and in a second state, the valve head and the flexible membrane are retracted substantially out of the valve cavity allowing fluid to flow through the valve assembly. In various implementations, the valve seat and/or the flexible membrane include an elastomer layer.
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公开(公告)号:EP3177708B1
公开(公告)日:2018-07-25
申请号:EP15757598.6
申请日:2015-08-05
CPC分类号: C12M27/00 , C12M21/08 , C12M23/12 , C12M27/18 , C12M29/10 , C12M29/12 , C12M29/18 , C12M41/44 , C12M41/48
摘要: Fluid circulation and leveling systems and methods of using the same are described. A fluid circulation system includes a fluid mixing chamber and open fluid chambers in fluid communication with the fluid mixing chamber. Each open fluid chamber includes a microfluidic fluid leveling conduit with an orifice disposed in the open fluid chamber at a minimum fluid level associated with a corresponding minimum fluid volume. A controller causes a first pump to generate a first direction of fluid flow during a first time period between the open fluid chambers, and causes the first pump to generate a second direction of fluid flow during a second time period between the first and second open fluid chambers. The controller also causes a second pump to generate a flow of fluid during a third time period from one of the first and second open fluid chambers into the fluid mixing chamber.
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公开(公告)号:EP3177708A1
公开(公告)日:2017-06-14
申请号:EP15757598.6
申请日:2015-08-05
CPC分类号: C12M27/00 , C12M21/08 , C12M23/12 , C12M27/18 , C12M29/10 , C12M29/12 , C12M29/18 , C12M41/44 , C12M41/48
摘要: Fluid circulation and leveling systems and methods of using the same are described. A fluid circulation system includes a fluid mixing chamber and open fluid chambers in fluid communication with the fluid mixing chamber. Each open fluid chamber includes a microfluidic fluid leveling conduit with an orifice disposed in the open fluid chamber at a minimum fluid level associated with a corresponding minimum fluid volume. A controller causes a first pump to generate a first direction of fluid flow during a first time period between the open fluid chambers, and causes the first pump to generate a second direction of fluid flow during a second time period between the first and second open fluid chambers. The controller also causes a second pump to generate a flow of fluid during a third time period from one of the first and second open fluid chambers into the fluid mixing chamber.
摘要翻译: 描述了流体循环和流平系统及其使用方法。 流体循环系统包括流体混合室和与流体混合室流体连通的开放流体室。 每个开放的流体室包括微流体流体调平导管,其具有以与相应的最小流体体积相关联的最小流体水平面设置在开放流体室中的孔口。 控制器使得第一泵在开放的流体室之间的第一时间段期间产生第一流体流动方向,并且使得第一泵在第一和第二开放流体之间的第二时间段期间产生第二流体流动方向 室。 控制器还使得第二泵在从第一和第二开放流体室之一进入流体混合室的第三时间段期间产生流体流。
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公开(公告)号:EP4326848A1
公开(公告)日:2024-02-28
申请号:EP22725591.6
申请日:2022-04-22
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公开(公告)号:EP4025684A1
公开(公告)日:2022-07-13
申请号:EP20790088.7
申请日:2020-09-04
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公开(公告)号:EP3831923A1
公开(公告)日:2021-06-09
申请号:EP20211221.5
申请日:2020-12-02
发明人: BORENSTEIN, Jeffrey , MOORE, Nathan , DOTY, Daniel , HAGGERTY, Timothy , CHAREST, Joseph , GIMBEL, Alla , MOTT, Vienna , ISENBERG, Brett , AZIZGOLSHANI, Hesham , CAIN, Brian , MESCHER, Mark
摘要: A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.
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