公开(公告)号：EP3458447A1

公开(公告)日：2019-03-27

申请号：EP17800051.9

申请日：2017-05-16

申请人： The Regents of the University of California ,  Fondazione Istituto Italiano di Tecnologia

发明人： PIOMELLI, Daniele ,  SCARPELLI, Rita ,  MIGLIORE, Marco ,  HEIM, Roger ,  GARCIA-GUZMÀN, Miguel

IPC分类号： C07D295/00

公开(公告)号：EP0804457A1

公开(公告)日：1997-11-05

申请号：EP95939898.0

申请日：1995-11-13

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： TSIEN, Roger, Y. ,  HEIM, Roger

IPC分类号： C12N15 ,  C07K14 ,  C12N5 ,  C12P21 ,  C12Q1 ,  G01N33

CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

公开(公告)号：EP0877805B1

公开(公告)日：2002-06-05

申请号：EP97905667.8

申请日：1997-01-31

申请人： The Regents of The University of California ,  Aurora Biosciences Corporation

发明人： TSIEN, Roger, Y. ,  HEIM, Roger ,  CUBITT, Andrew

IPC分类号： C12N15/12 ,  C12N15/62 ,  C12N15/70 ,  C12N15/85 ,  C12N5/10 ,  C12N1/21 ,  C07K14/435 ,  C12Q1/37 ,  G01N33/553

CPC分类号： C12Q1/485 ,  C07K14/43595 ,  C07K2319/00 ,  C12Q1/37 ,  G01N33/542 ,  G01N2333/43595

公开(公告)号：EP0886644B1

公开(公告)日：2004-10-20

申请号：EP97941350.7

申请日：1997-08-15

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  The STATE OF OREGON acting by and through the STATE BOARD OF HIGHER EDUCATION on behalf of the UNIV. OF OREGON ,  INVITROGEN CORPORATION

发明人： TSIEN, Roger, Y. ,  CUBITT, Andrew, B. ,  HEIM, Roger ,  ORMO, Mats, F. ,  REMINGTON, James, S.

IPC分类号： C07H21/04 ,  C07K14/00 ,  C07K16/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

CPC分类号： C07K14/43595 ,  Y10S435/968 ,  Y10T436/2525

摘要： Engineered fluorescent proteins, nucleic acids encoding them and methods of use.

公开(公告)号：EP0804457B1

公开(公告)日：2001-06-06

申请号：EP95939898.3

申请日：1995-11-13

申请人： The Regents of the University of California

发明人： TSIEN, Roger, Y. ,  HEIM, Roger

IPC分类号： C07H21/04 ,  C07K14/435 ,  C12Q1/25 ,  C12Q1/68

CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

公开(公告)号：EP0877805A1

公开(公告)日：1998-11-18

申请号：EP97905667.0

申请日：1997-01-31

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  Aurora Biosciences Corporation

发明人： TSIEN, Roger, Y. ,  HEIM, Roger ,  CUBITT, Andrew

IPC分类号： C07K14 ,  C12N1 ,  C12N15 ,  C12Q1 ,  G01N33

CPC分类号： C12Q1/485 ,  C07K14/43595 ,  C07K2319/00 ,  C12Q1/37 ,  G01N33/542 ,  G01N2333/43595

摘要： This invention provides tandem fluorescent protein constructs including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.