公开(公告)号：EP2776463A1

公开(公告)日：2014-09-17

申请号：EP12847241.2

申请日：2012-11-07

申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services

发明人： CONNORS, Mark ,  HUANG, Jinghe ,  LAUB, Leo, B. ,  KWONG, Peter ,  NABEL, Gary ,  MASCOLA, John, R. ,  ZHANG, Baoshan ,  RUDICELL, Rebecca, S. ,  GEORGIEV, Ivelin ,  YANG, Yongping ,  ZHU, Jiang ,  OFEK, Gilad

IPC分类号： C07K16/10 ,  A61K39/42 ,  A61P31/18 ,  C12N15/13

CPC分类号： C07K16/1063 ,  A61K39/42 ,  A61K45/06 ,  A61K49/0004 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/1045 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  G01N33/56972 ,  G01N33/56983 ,  G01N33/56988 ,  G01N2333/161

摘要： Monoclonal neutralizing antibodies are disclosed that specifically bind to the HIV-1 gp41 membrane-proximal external region (MPER). Also disclosed are compositions including the disclosed antibodies that specifically bind gp41, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of HIV-1 in a biological sample, or detecting an HIV-1 infection or diagnosing AIDS in a subject. In additional, the broad neutralization breadth of the disclosed antibodies makes them ideal for treating a subject with an HIV infection. Thus, disclosed are methods of treating and/or preventing HIV infection.

摘要翻译： 公开了特异性结合HIV-1 gp41膜近端外部区域（MPER）的单克隆中和抗体。 还公开了包括所公开的特异性结合gp41的抗体，编码这些抗体的核酸，包括核酸的表达载体和表达核酸的分离的宿主细胞的组合物。 本文公开的抗体和组合物可用于检测生物样品中HIV-1的存在，或检测受试者中的HIV-1感染或AIDS的诊断。 此外，所公开抗体的广泛中和宽度使其成为治疗HIV感染的受试者的理想选择。 因此，公开了治疗和/或预防HIV感染的方法。

公开(公告)号：EP2707388B1

公开(公告)日：2019-06-12

申请号：EP12782672.5

申请日：2012-03-23

申请人： Duke University ,  The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services

发明人： MASCOLA, John, R. ,  NABEL, Gary ,  HAYNES, Barton, F. ,  WU, Xueling ,  KEPLER, Thomas, B. ,  KWONG, Peter

IPC分类号： C07K16/10 ,  C12N15/13 ,  A61K39/42 ,  A61P31/18

公开(公告)号：EP2776463B1

公开(公告)日：2017-09-20

申请号：EP12847241.2

申请日：2012-11-07

申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services

发明人： CONNORS, Mark ,  HUANG, Jinghe ,  LAUB, Leo, B. ,  KWONG, Peter ,  NABEL, Gary ,  MASCOLA, John, R. ,  ZHANG, Baoshan ,  RUDICELL, Rebecca, S. ,  GEORGIEV, Ivelin ,  YANG, Yongping ,  ZHU, Jiang ,  OFEK, Gilad

IPC分类号： C07K16/10 ,  A61K39/42 ,  A61P31/18 ,  C12N15/13

CPC分类号： C07K16/1063 ,  A61K39/42 ,  A61K45/06 ,  A61K49/0004 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/1045 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  G01N33/56972 ,  G01N33/56983 ,  G01N33/56988 ,  G01N2333/161

摘要： Monoclonal neutralizing antibodies are disclosed that specifically bind to the HIV-1 gp41 membrane-proximal external region (MPER). Also disclosed are compositions including the disclosed antibodies that specifically bind gp41, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of HIV-1 in a biological sample, or detecting an HIV-1 infection or diagnosing AIDS in a subject. In additional, the broad neutralization breadth of the disclosed antibodies makes them ideal for treating a subject with an HIV infection. Thus, disclosed are methods of treating and/or preventing HIV infection.

摘要翻译： 公开了单克隆中和抗体，其特异性结合HIV-1 gp41膜近端外部区域（MPER）。 还公开了包含特异性结合gp41的公开抗体，编码这些抗体的核酸，包含核酸的表达载体和表达核酸的分离的宿主细胞的组合物。 本文公开的抗体和组合物可用于检测生物样品中HIV-1的存在，或检测受试者中的HIV-1感染或诊断AIDS。 另外，所公开的抗体的广泛中和宽度使得它们理想地用于治疗HIV感染的受试者。 因此，公开了治疗和/或预防HIV感染的方法。

公开(公告)号：EP3525821A1

公开(公告)日：2019-08-21

申请号：EP17862065.4

申请日：2017-10-17

申请人： University of Maryland ,  The United States Of America, As Represented By The Secretary, Department Of Health And Human Services ,  Guenaga, Javier

发明人： GUENAGA, Javier ,  LI, Yuxing ,  STEINHARDT, James ,  MASCOLA, John, R.

IPC分类号： A61K39/395 ,  C07K16/46 ,  C07K16/10 ,  A61P31/18

公开(公告)号：EP2480572B1

公开(公告)日：2019-01-30

申请号：EP10760878.8

申请日：2010-09-24

申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services ,  University of Washington through its Center for Commercialization

发明人： MASCOLA, John, R. ,  WYATT, Richard ,  WU, Xueling ,  LI, Yuxing ,  HOGERKORP, Carl-Magnus ,  ROEDERER, Mario ,  YANG, Zhi-yong ,  NABEL, Gary ,  KWONG, Peter ,  ZHOU, Tongqing ,  CONNORS, Mark ,  OFEK, Gilad ,  SHAPIRO, Lawrence ,  ZHU, Jiang ,  SCHIEF, William R.

IPC分类号： C07K16/10

公开(公告)号：EP2828294A1

公开(公告)日：2015-01-28

申请号：EP13763664.3

申请日：2013-03-15

申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services

发明人： MASCOLA, John, R. ,  LYNCH, Rebecca, M. ,  WU, Xueling ,  CONNORS, Mark ,  ZHOU, Tongqing ,  CASAZZA, Joseph ,  KWONG, Peter, D.

IPC分类号： C07K16/10 ,  A61K39/42 ,  A61P31/18

CPC分类号： C07K16/1063 ,  A61K39/42 ,  A61K45/06 ,  A61K49/0004 ,  A61K2039/505 ,  A61K2039/545 ,  C07K2317/31 ,  C07K2317/76 ,  G01N33/56983

摘要： Neutralizing antibodies that specifically bind to HIV-1 gpl20 and antigen binding fragments of these antibodies are disclosed. Nucleic acids encoding these antibodies, vectors and host cells are also provided. Methods for detecting HIV using these antibodies are disclosed. In addition, the use of these antibodies, antigen binding fragment, nucleic acids and vectors to prevent and/or treat an HIV infection is disclosed.

公开(公告)号：EP2707388A2

公开(公告)日：2014-03-19

申请号：EP12782672.5

申请日：2012-03-23

申请人： Duke University ,  The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services

发明人： MASCOLA, John, R. ,  NABEL, Gary ,  HAYNES, Barton, F. ,  WU, Xueling ,  KEPLER, Thomas, B. ,  KWONG, Peter

IPC分类号： C07K16/10 ,  C12N15/13 ,  A61K39/42 ,  A61P31/18

CPC分类号： C07K16/1063 ,  A61K39/42 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/76 ,  G01N33/56988

摘要： Antibody VRC01 represents a human immunoglobulin that neutralizes—˜90% of diverse HIV-1 isolates. To understand how such broadly neutralizing HIV-1 antibodies develop and recognize the viral envelope, we used X-ray crystallography and 454 pyrosequencing to characterize additional antibodies from HIV-1-infected individuals. Crystal structures revealed a convergent mode of binding of different antibodies to the same CD4-binding-site epitope. Antibody recognition was achieved through the evolution of complementary contact domains that were generated in diverse ways. Phylogenetic analysis of expressed heavy and light chains determined by deep sequencing revealed a common pathway of antibody heavy chain maturation confined to IGHV1-2*02 lineage that could pair with different light chains. The maturation pathway inferred by antibodyomics reveals that diverse antibodies evolve to a highly affinity-matured state to recognize an invariant viral structure, providing insight into the development and evolution of broadly neutralizing HIV-1 immunity.