公开(公告)号：EP2998320B1

公开(公告)日：2018-07-18

申请号：EP15188264.4

申请日：2012-04-19

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： HO, Mitchell ,  FENG, Mingqian ,  GAO, Wei ,  KIM, Heungnam ,  DIMITROV, Dimiter S.,

IPC分类号： C07K16/30 ,  A61K39/395 ,  C07K16/28 ,  G01N33/574 ,  G01N33/68 ,  A61K39/00 ,  C07K14/21 ,  A61K47/68 ,  A61P35/00

CPC分类号： C07K16/3076 ,  A61K39/39558 ,  A61K47/6803 ,  A61K47/6813 ,  A61K47/6829 ,  A61K47/6849 ,  A61K47/6851 ,  A61K2039/505 ,  C07K14/21 ,  C07K16/28 ,  C07K16/303 ,  C07K16/3092 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/33 ,  C07K2319/55 ,  G01N33/57438 ,  G01N33/57492 ,  G01N33/6893 ,  G01N2333/705 ,  G01N2400/40

摘要： Described herein is the identification of human monoclonal antibodies that bind GPC3 or heparan sulfate (HS) chains on GPC3 with high affinity. The antibodies described herein are capable of inhibiting HCC cell growth and migration. Provided are human monoclonal antibodies specific for GPC3 or HS chains on GPC3, including immunoglobulin molecules, such as IgG antibodies, as well as antibody fragments, such as single-domain VH antibodies or single chain variable fragments (scFv). Further provided are compositions including the antibodies that bind GPC3 or HS chains on GPC3, nucleic acid molecules encoding these antibodies, expression vectors comprising the nucleic acids, and isolated host cells that express the nucleic acids. Methods of treating cancer and/or inhibiting tumor growth or metastasis are also provided. Further provided are methods of detecting cancer in a subject and confirming a diagnosis of cancer in a subject.

公开(公告)号：EP2699603B1

公开(公告)日：2016-03-02

申请号：EP12717009.0

申请日：2012-04-19

申请人： The United States of America As Represented by the Secretary Department of Health and Human Services

发明人： HO, Mitchell ,  FENG, Mingqian ,  GAO, Wei ,  KIM, Heungnam ,  DIMITROV, Dimiter, S.

IPC分类号： C07K16/30 ,  A61K39/395 ,  G01N33/68 ,  A61K39/00

CPC分类号： C07K16/3076 ,  A61K39/39558 ,  A61K47/6803 ,  A61K47/6813 ,  A61K47/6829 ,  A61K47/6849 ,  A61K47/6851 ,  A61K2039/505 ,  C07K14/21 ,  C07K16/28 ,  C07K16/303 ,  C07K16/3092 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/33 ,  C07K2319/55 ,  G01N33/57438 ,  G01N33/57492 ,  G01N33/6893 ,  G01N2333/705 ,  G01N2400/40

摘要： Described herein is the identification of human monoclonal antibodies that bind GPC3 or heparan sulfate (HS) chains on GPC3 with high affinity. The antibodies described herein are capable of inhibiting HCC cell growth and migration. Provided are human monoclonal antibodies specific for GPC3 or HS chains on GPC3, including immunoglobulin molecules, such as IgG antibodies, as well as antibody fragments, such as single-domain VH antibodies or single chain variable fragments (scFv). Further provided are compositions including the antibodies that bind GPC3 or HS chains on GPC3, nucleic acid molecules encoding these antibodies, expression vectors comprising the nucleic acids, and isolated host cells that express the nucleic acids. Methods of treating cancer and/or inhibiting tumor growth or metastasis are also provided. Further provided are methods of detecting cancer in a subject and confirming a diagnosis of cancer in a subject.

公开(公告)号：EP2998320A1

公开(公告)日：2016-03-23

申请号：EP15188264.4

申请日：2012-04-19

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： HO, Mitchell ,  FENG, Mingqian ,  GAO, Wei ,  KIM, Heungnam ,  DIMITROV, Dimiter S.,

IPC分类号： C07K16/30 ,  A61K39/395 ,  A61K47/48 ,  C07K16/28 ,  G01N33/574 ,  G01N33/68 ,  A61K39/00

CPC分类号： C07K16/3076 ,  A61K39/39558 ,  A61K47/6803 ,  A61K47/6813 ,  A61K47/6829 ,  A61K47/6849 ,  A61K47/6851 ,  A61K2039/505 ,  C07K14/21 ,  C07K16/28 ,  C07K16/303 ,  C07K16/3092 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/33 ,  C07K2319/55 ,  G01N33/57438 ,  G01N33/57492 ,  G01N33/6893 ,  G01N2333/705 ,  G01N2400/40

摘要： Described herein is the identification of human monoclonal antibodies that bind GPC3 or heparan sulfate (HS) chains on GPC3 with high affinity. The antibodies described herein are capable of inhibiting HCC cell growth and migration. Provided are human monoclonal antibodies specific for GPC3 or HS chains on GPC3, including immunoglobulin molecules, such as IgG antibodies, as well as antibody fragments, such as single-domain VH antibodies or single chain variable fragments (scFv). Further provided are compositions including the antibodies that bind GPC3 or HS chains on GPC3, nucleic acid molecules encoding these antibodies, expression vectors comprising the nucleic acids, and isolated host cells that express the nucleic acids. Methods of treating cancer and/or inhibiting tumor growth or metastasis are also provided. Further provided are methods of detecting cancer in a subject and confirming a diagnosis of cancer in a subject.

摘要翻译： 本文描述了鉴定以高亲和力在GPC3上结合GPC3或硫酸乙酰肝素（HS）链的人单克隆抗体。 本文所述的抗体能够抑制HCC细胞生长和迁移。 提供了对GPC3上的GPC3或HS链特异性的人单克隆抗体，包括免疫球蛋白分子如IgG抗体，以及抗体片段，如单结构域VH抗体或单链可变片段（scFv）。 进一步提供了包含GPC3上结合GPC3或HS链的抗体，编码这些抗体的核酸分子，包含核酸的表达载体和表达核酸的分离的宿主细胞的组合物。 还提供了治疗癌症和/或抑制肿瘤生长或转移的方法。 还提供了检测受试者中的癌症并确认受试者中癌症的诊断的方法。

公开(公告)号：EP2699603A1

公开(公告)日：2014-02-26

申请号：EP12717009.0

申请日：2012-04-19

申请人： The United States of America As Represented by the Secretary Department of Health and Human Services

发明人： HO, Mitchell ,  FENG, Mingqian ,  GAO, Wei ,  KIM, Heungnam ,  DIMITROV, Dimiter, S.

IPC分类号： C07K16/30

CPC分类号： C07K16/3076 ,  A61K39/39558 ,  A61K47/6803 ,  A61K47/6813 ,  A61K47/6829 ,  A61K47/6849 ,  A61K47/6851 ,  A61K2039/505 ,  C07K14/21 ,  C07K16/28 ,  C07K16/303 ,  C07K16/3092 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/33 ,  C07K2319/55 ,  G01N33/57438 ,  G01N33/57492 ,  G01N33/6893 ,  G01N2333/705 ,  G01N2400/40

摘要： Described herein is the identification of human monoclonal antibodies that bind GPC3 or heparan sulfate (HS) chains on GPC3 with high affinity. The antibodies described herein are capable of inhibiting HCC cell growth and migration. Provided are human monoclonal antibodies specific for GPC3 or HS chains on GPC3, including immunoglobulin molecules, such as IgG antibodies, as well as antibody fragments, such as single-domain VH antibodies or single chain variable fragments (scFv). Further provided are compositions including the antibodies that bind GPC3 or HS chains on GPC3, nucleic acid molecules encoding these antibodies, expression vectors comprising the nucleic acids, and isolated host cells that express the nucleic acids. Methods of treating cancer and/or inhibiting tumor growth or metastasis are also provided. Further provided are methods of detecting cancer in a subject and confirming a diagnosis of cancer in a subject.