公开(公告)号：EP2714884A1

公开(公告)日：2014-04-09

申请号：EP11867040.5

申请日：2011-05-27

申请人： The University of British Columbia

发明人： HANSEN, Carl Lars Genghis ,  VANINSBERGHE, Michael ,  WHITE, Adam ,  PETRIV, Oleh ,  LEAVER, Tim ,  SINGHAL, Anupam ,  BOWDEN, William ,  LECAULT, Veronique ,  DA COSTA, Dan ,  WU, Leo ,  RUSSELL, Georgia ,  SIKORSKI, Darek

IPC分类号： C12M1/00 ,  C12M3/00 ,  C12N1/00 ,  C12N5/07 ,  C12Q1/02 ,  C12Q1/68 ,  G01N1/28 ,  G01N33/50

CPC分类号： C12Q1/6806 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/086 ,  C12M23/16 ,  C12Q1/6844 ,  G01N1/34 ,  G01N33/5005 ,  G01N33/54366 ,  G01N33/569

摘要： Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

公开(公告)号：EP2714884B1

公开(公告)日：2019-08-21

申请号：EP11867040.5

申请日：2011-05-27

申请人： The University of British Columbia

发明人： HANSEN, Carl Lars Genghis ,  VANINSBERGHE, Michael ,  WHITE, Adam ,  PETRIV, Oleh ,  LEAVER, Tim ,  SINGHAL, Anupam ,  BOWDEN, William ,  LECAULT, Veronique ,  DA COSTA, Dan ,  WU, Leo ,  RUSSELL, Georgia ,  SIKORSKI, Darek

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/6844 ,  B01L7/00