公开(公告)号：EP4001425A1

公开(公告)日：2022-05-25

申请号：EP19938680.6

申请日：2019-07-19

申请人： Tokyo Electron Limited ,  SHIMADZU CORPORATION ,  Foundation for Biomedical Research and Innovation at Kobe

发明人： HARA Keisuke ,  GOMI Shinichi ,  NAGATA Tomohisa ,  KAGAWA Kenichi ,  OSHIMA Yasuhiro ,  SUZUKI Takashi ,  TAKAHASHI Masatoshi ,  YAMAMOTO Takako ,  KAWAMATA Shin

IPC分类号： C12Q1/06 ,  C12N5/071 ,  C12N5/0735 ,  G01N33/50 ,  G01N33/68

摘要： The present invention relates to a method for evaluating the state of cell differentiation during the process of inducing differentiation of human induced pluripotent stem cells (iPS cells) into retinal pigment epithelial cells. The present invention further relates to a method for evaluating the state of cell differentiation during the process of inducing differentiation of human embryonic stem cells (ES cells) into retinal pigment epithelial cells.

公开(公告)号：EP3202911A1

公开(公告)日：2017-08-09

申请号：EP15848017.8

申请日：2015-09-29

申请人： Tokyo Electron Limited

发明人： HATABAYASHI Kunitada ,  TOMITA Kentaro ,  GOMI Shinichi ,  KURAKAZU Tomoaki ,  OSHIMA Yasuhiro ,  KAGAWA Kenichi ,  OZAKI Shigenori

IPC分类号： C12Q1/02 ,  C12M1/34 ,  C12M3/00 ,  C12N5/071

CPC分类号： C12M41/46 ,  C12M41/36 ,  C12M41/48 ,  C12N5/0606 ,  C12N5/0696 ,  C12Q1/02 ,  G01N33/5073

摘要： There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

摘要翻译： 提供了一种不依赖熟练技术人员的判断而容易地确定多能干细胞的未分化状态的方法。 该方法包括：基于培养多能干细胞的培养基中含有的细胞外代谢物的变异值的时间依赖性变化，评价多能干细胞的未分化状态的步骤，其中细胞外代谢物处于 选自由L-谷氨酸，L-丙氨酸和氨组成的组中的至少一种。

公开(公告)号：EP3130660A1

公开(公告)日：2017-02-15

申请号：EP15767900.2

申请日：2015-03-24

申请人： Tokyo Electron Limited

发明人： GOMI Shinichi ,  OSHIMA Yasuhiro ,  KAGAWA Kenichi ,  KURAKAZU Tomoaki ,  GOMI Hisashi ,  OZAKI Shigenori

IPC分类号： C12M1/42 ,  C12N1/00 ,  C12N5/071 ,  C12N5/10 ,  C12N13/00

CPC分类号： C12M47/02 ,  C12M25/02 ,  C12M33/00 ,  C12M33/08 ,  C12N1/02 ,  C12N13/00

摘要： A cell separation device (140) imparts oscillation from a back-face side to a cell culture film (77) to which cells adhere to the surface thereof and thereby separates a cell from the cell culture film (77). The cell separation device (140) is provided with an ultrasonic probe (141) having an oscillator (141 a) for ultrasonically oscillating, a frame (142) disposed so as to surround the oscillator (141a), and a first detection unit (143) for detecting a force applied to the frame (142) in a predetermined direction. The oscillator (141a) imparts ultrasonic oscillation selectively to a predetermined cell adhering to the surface of the cell culture film (77). The frame (142) is pushed in until the force applied to the frame (142) from the back face of the cell culture film (77) reaches a predetermined magnitude on the basis of the result of detection by the first detection unit (143), and the oscillator (141a) then ultrasonically oscillates in a state in which the frame (142) abuts the back face of the cell culture film (77).

摘要翻译： 细胞分离装置（140）将背面侧的振动赋予细胞粘着于其表面的细胞培养膜（77），从而将细胞与细胞培养膜（77）分离。 细胞分离装置（140）具有超声波探头（141），该超声波探头具有用于超声波振荡的振荡器（141a），以围绕振荡器（141a）的方式设置的框架（142）和第一检测单元 ），用于检测在预定方向上施加到框架（142）的力。 振荡器（141a）选择性地对附着于细胞培养膜（77）的表面的规定的细胞施加超声波振动。 基于第一检测单元（143）的检测结果，将框架（142）推入直到从细胞培养膜（77）的背面施加到框架（142）的力达到预定量值， ，并且在框架（142）抵靠细胞培养膜（77）的背面的状态下，振荡器（141a）超声波振荡。

公开(公告)号：EP3109312A1

公开(公告)日：2016-12-28

申请号：EP15752598.1

申请日：2015-02-16

申请人： Tokyo Electron Limited

发明人： KURAKAZU Tomoaki ,  OSHIMA Yasuhiro ,  KIMURA Yoshio ,  KAGAWA Kenichi ,  GOMI Shinichi ,  OZAKI Shigenori

IPC分类号： C12M1/00 ,  C12M3/00

CPC分类号： C12M23/12 ,  C12M23/04 ,  C12M23/20 ,  C12M23/24 ,  C12M25/00 ,  C12M25/06 ,  C12M29/10

摘要： A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

摘要翻译： 一种细胞培养容器，其特征在于，在容器主体的一个表面上装有容器主体和平板，其特征在于，容器主体配备有流入容器主体的流入口， 从流入口流入容器主体的液体可以通过的通道，以及通过通道的液体可以从容器主体流出的流出口，其中，在通道的底面上 通过通道的细胞可以接种的多个细胞播种区域沿着通道并排布置。