公开(公告)号：EP2110662A1

公开(公告)日：2009-10-21

申请号：EP08710799.1

申请日：2008-02-05

Applicant: Toppan Printing Co., Ltd.

Inventor： NAKAYAMA, Masato

IPC: G01N27/416 ,  G01N27/26 ,  G01N27/327 ,  G01N27/48 ,  G01N37/00

CPC classification number: G01N27/3277 ,  B01L3/502715 ,  B01L7/52 ,  C12Q1/6825 ,  C12Q2537/101 ,  C12Q2563/113 ,  C12Q2565/607

Abstract: A biomolecules detection method which detects a detection target biomolecule by a measurement of an electrochemical response, the method including: a standard solution measurement process which measures an electrochemical response of a standard solution which includes an electrochemically reactive agent having a direct or indirect binding capacity to the detection target biomolecule; sample solution measurement process which measures the electrochemical response of a sample solution, the sample solution containing, a sample material containing the detection target biomolecule, and the electrochemically reactive agent; a comparison process which compares an electrochemical response measurement result of the standard solution and an electrochemical response measurement result of the sample solution, and thereby obtains a comparison result; and a biomolecules content information detection process which, based on the comparison result, detects a content information of the detection target biomolecule in the sample solution.

Abstract translation: 一种生物分子检测方法，其通过电化学响应的测量来检测检测目标生物分子，所述方法包括：标准溶液测量方法，其测量标准溶液的电化学响应，所述标准溶液包括具有直接或间接结合能力的电化学反应剂 检测目标生物分子; 样品溶液测量过程，其测量样品溶液的电化学响应，样品溶液含有含有检测目标生物分子的样品材料和电化学反应剂; 比较标准溶液的电化学响应测量结果和样品溶液的电化学响应测量结果的比较过程，从而获得比较结果; 以及基于比较结果的检测目标生物分子的内容信息的生物分子内容信息检测处理。

公开(公告)号：EP2873976A1

公开(公告)日：2015-05-20

申请号：EP13814000.9

申请日：2013-07-02

Applicant: Toppan Printing Co., Ltd. ,  Tokyo Institute of Technology

Inventor： HIRASE, Takumi ,  NAKAYAMA, Masato ,  HANDA, Hiroshi ,  SAKAMOTO, Satoshi ,  NAITO, Yasuyuki

IPC: G01N33/543 ,  G01N21/64

CPC classification number: G01N33/54326 ,  G01N21/6428 ,  G01N21/645

Abstract: A detection system of a test substance (10), includes: a magnetic body (2) that is modified by a first substance (3) capable of being bound to the test substance (10) at a first binding site of the test substance; a marker (4) that is modified by a second substance (3) capable of being bound to the test substance (10) at a second binding site of the test substance (10), the second binding site being a different site from the first binding site; a development medium (20) that has a flow passage (24) into which liquid, in which the magnetic body (2) and the marker (4) is dispersed, flows by a capillary phenomenon, the development medium having a detection position for detecting the marker (4) in a portion of the flow passage (24), the development medium having light transparency at least in the detection position; and a magnetic force generating portion (30) that is equipped in the development medium (20) in order to hold the magnetic body (2) in a portion of the flow passage (24).

Abstract translation: 测试物质（10）的检测系统包括：被测物质的第一结合位点被能够与测试物质（10）结合的第一物质（3）改性的磁体（2）; 由能够在被检物质（10）的第二结合位点与被检物质（10）结合的第二物质（3）修饰的标记物（4），所述第二结合位点是与所述第一物质 结合位点; 具有流路（24）的显影介质（20），其中分散有磁性体（2）和标记物（4）的液体通过毛细管现象流动，所述显影介质具有检测位置 在所述流路（24）的一部分中的所述标记（4），所述显影介质至少在所述检测位置具有透光性; 以及设置在显影介质（20）中以将磁体（2）保持在流路（24）的一部分中的磁力产生部分（30）。

公开(公告)号：EP2551356A1

公开(公告)日：2013-01-30

申请号：EP11759408.5

申请日：2011-03-23

Applicant: Toppan Printing Co., Ltd.

Inventor： MAKINO, Yoichi ,  YAMANE, Akio ,  NAKAYAMA, Masato

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2537/161 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2535/125 ,  C12Q2549/101 ,  C12Q2563/107 ,  C12Q2565/101

Abstract: Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.

Abstract translation: 本发明公开了一种检测具有多态碱基的目标碱基序列的方法，该方法包括：（a）向具有包含目标碱基序列的碱基序列的靶核酸的核酸样品中添加至少一种 检测引物的类型，至少一种类型的竞争性引物和至少一种类型的共同引物; （b）以竞争的方式将检测引物和竞争性引物退火至靶核酸，由此合成延伸产物A; （c）将通用引物退火到步骤（b）或后续步骤（d）中获得的延伸产物A的步骤，从而合成延伸产物B; （d）将检测引物或竞争性引物退火到在前面步骤（c）中获得的延伸产物B的步骤，从而合成延伸产物A; 和（e）检测扩展产品A或扩展产品B的步骤。

公开(公告)号：EP2551356B1

公开(公告)日：2017-06-14

申请号：EP11759408.5

申请日：2011-03-23

Applicant: Toppan Printing Co., Ltd.

Inventor： MAKINO, Yoichi ,  YAMANE, Akio ,  NAKAYAMA, Masato

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2537/161 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2535/125 ,  C12Q2549/101 ,  C12Q2563/107 ,  C12Q2565/101