公开(公告)号：EP2836604A4

公开(公告)日：2016-04-13

申请号：EP13775787

申请日：2013-04-08

申请人： UNIV COLUMBIA ,  US GOVERNMENT

发明人： JU JINGYUE ,  KUMAR SHIV ,  TAO CHUANJUAN ,  CHIEN MINCHEN ,  RUSSO JAMES J ,  KASIANOVICZ JOHN J ,  ROBERTSON JOSEPH W F

IPC分类号： C12Q1/42

CPC分类号： C12Q1/6883 ,  B82Y5/00 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/6869 ,  C12Q2600/158 ,  G01N33/48721 ,  G01N2333/9125 ,  C12Q2565/631 ,  C12Q2521/525 ,  C12Q2521/543 ,  C12Q2535/107

摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

公开(公告)号：EP2652153A4

公开(公告)日：2014-05-14

申请号：EP11848220

申请日：2011-12-16

申请人： UNIV COLUMBIA

发明人： JU JINGYUE ,  KUMAR SHIV ,  LI ZENGMIN ,  TAO CHUANJUAN ,  CHIEN MINCHEN ,  RUSSO JAMES J ,  KALACHIKOV SERGEY ,  SHEPARD KEN ,  ROSENSTEIN JACOB KARL

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H21/00 ,  G01N33/48721 ,  C12Q2525/113 ,  C12Q2525/117 ,  C12Q2565/518 ,  C12Q2565/631

公开(公告)号：EP3105354A4

公开(公告)日：2018-02-14

申请号：EP15749646

申请日：2015-02-12

申请人： UNIV COLUMBIA

发明人： TAO CHUANJUAN ,  KUMAR SHIV ,  CHIEN MINCHEN ,  JU JINGYUE

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q1/6853 ,  C12Q2525/186 ,  C12Q2565/631 ,  C12Q2535/113

摘要： This invention provides methods of using labeled primers or probes for nucleic acid target detection and to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, and sets of oligonucleotide primers for use in such methods, as well as methods of quantitative PCR coupled with nanopore detection.