公开(公告)号：EP3116989A4

公开(公告)日：2017-08-16

申请号：EP15761253

申请日：2015-03-10

申请人： UNIV JOHNS HOPKINS

发明人： SMITH CHRISTOPHER ,  LEVCHENKO ANDRE ,  KIM DEOK-HO ,  QUINONES-HINOJOSA ALFREDO

IPC分类号： C12M1/34 ,  B01L3/00 ,  C12Q1/02 ,  G01N33/50

CPC分类号： G01N33/5029 ,  B01L3/5085 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0896 ,  B01L2300/163 ,  B01L2400/086 ,  G01N15/00 ,  G01N33/4833 ,  G01N33/5005 ,  G01N33/574 ,  G01N33/57407 ,  G01N2015/0003

摘要： The present invention is directed to creating a highly versatile, high throughput, and convenient platform for interrogating migration phenotypes of GB cells in the context of diverse environmental parameters. Specifically, engineered substrates are employed to mimic the mechanical signals of natural ECM topography, and combine these tools with chemical cues presented in soluble and immobilized forms (PDGF and laminin, respectively). This platform achieves far greater resolution and sensitivity in migration analyses than do commonly used methods. More importantly, it can provide highly informative, patient specific results regarding tumor progression in vivo. Furthermore these capabilities strongly convey the immense clinical, prognostic potential of this simple test.

摘要翻译： 本发明旨在创建用于在不同环境参数的背景下询问GB细胞的迁移表型的高度通用，高通量和便利的平台。 具体而言，工程基板被用来模仿天然ECM地形的机械信号，并将这些工具与可溶性和固定化形式（分别为PDGF和层粘连蛋白）呈现的化学线索组合。 该平台在迁移分析中的分辨率和灵敏度远远高于常用方法。 更重要的是，它可以提供关于体内肿瘤进展的高信息性，患者特异性结果。 此外，这些功能强有力地传达了这个简单测试的巨大临床和预后潜力。