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    METHOD OF NUCLEAR REPROGRAMMING
    3.
    发明公开
    METHOD OF NUCLEAR REPROGRAMMING 审中-公开
    NUKLEAR-NEUPROGRAMMIERUNGSVERFAHREN

    公开(公告)号：EP2268809A4

    公开(公告)日：2013-02-27

    申请号：EP09738908

    申请日：2009-05-01

    申请人： UNIV KYOTO

    发明人： YAMANAKA SHINYA ,  OKITA KEISUKE

    IPC分类号： C12N15/09 ,  C12N5/074

    CPC分类号： C12N5/0696 ,  C12N15/79 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12N2506/13 ,  C12N2506/1307 ,  C12N2506/1361 ,  C12N2510/00

    摘要： This invention provides a method of producing an induced pluripotent stem cell comprising the step of introducing at least one kind of non-viral expression vector (more preferably a plasmid vector) incorporating at least one gene that encodes a reprogramming factor into a somatic cell. An induced pluripotent stem cell wherein no exogenous genes induced is integrated into the cellular genome is also provided.

    摘要翻译： 产生诱导性多能干细胞的方法包括将一种或多种非病毒表达载体引入体细胞中。 载体包括Oct家族基因，Klf家族基因，Sox家族基因，Myc家族基因，Lin家族基因和Nanog基因中的一种或多种。 然后将体细胞在支持多能干细胞的培养基中培养。 一种或多种引入的非病毒表达载体的至少一部分基本上不整合到染色体中。

    METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
    4.
    发明公开
    METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 审中-公开
    VERFAHREN ZUR EFFIZIENTEN HERSTELLUNG INDUZIERTER PLURIPOTENTER STAMMZELLEN

    公开(公告)号：EP2462225A4

    公开(公告)日：2013-12-18

    申请号：EP10806584

    申请日：2010-08-06

    申请人： UNIV KYOTO

    发明人： OKITA KEISUKE ,  NAKAGAWA MASATO ,  YAMANAKA SHINYA

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N15/85

    CPC分类号： C12N15/85 ,  C12N5/0696 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/13 ,  C12N2506/1361 ,  C12N2510/00 ,  C12N2800/108 ,  C12N2800/30

    摘要： Provided is a method of producing an iPS cell, comprising bringing (a) Oct3/4 or a nucleic acid that encodes the same, (b) Klf4 or a nucleic acid that encodes the same, and (c) Sox2 or a nucleic acid that encodes the same, as well as (d1) L-Myc or a nucleic acid that encodes the same and/or (d2) a functional inhibitor of p53, into contact with a somatic cell. It is preferable that (a) a nucleic acid that encodes Oct3/4, (b) a nucleic acid that encodes Klf4, (c) a nucleic acid that encodes Sox2, (d1) a nucleic acid that encodes L-Myc and (e) a nucleic acid that encodes Lin28 or Lin28b be inserted into an episomal vector having loxP sequences placed in the same orientation on the 5′ and 3′ sides of a vector constituent essential for the replication of the vector, that (d2) a nucleic acid that encodes an shRNA against p53 be inserted into a vector ensuring transient expression (plasmid vector and the like), and that all these nucleic acids be transferred to a somatic cell.

    摘要翻译： 本发明提供生产iPS细胞的方法，其包括使（a）Oct3 / 4或其编码核酸，（b）Klf4或编码其的核酸，和（c）Sox2或 （d1）L-Myc或编码它们的核酸和/或（d2）p53功能性抑制剂编码相同的基因，使其与体细胞接触。 （a）编码Oct3 / 4的核酸，（b）编码Klf4的核酸，（c）编码Sox2的核酸，（d1）编码L-Myc的核酸和（e） ）将编码Lin28或Lin28b的核酸插入具有在5'端以相同方向放置的loxP序列的游离型载体中， 和3' 将（d2）编码针对p53的shRNA的核酸插入到保证瞬时表达的载体（质粒载体等）中，并转移所有这些核酸 到体细胞。