公开(公告)号：EP4425178A2

公开(公告)日：2024-09-04

申请号：EP24176389.5

申请日：2017-05-03

申请人： University Health Network ,  Sinai Health System

发明人： DINIZ DE CARVALHO, Daniel ,  SHEN, Shu Yi ,  SINGHANIA, Rajat

IPC分类号： G01N33/53

CPC分类号： C12N15/10 ,  C12N15/1093 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6886 ,  G01N33/5308 ,  C12Q2600/15420130101 ,  C12N15/1003

摘要： There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 ng of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.

公开(公告)号：EP3704267A1

公开(公告)日：2020-09-09

申请号：EP18874092.2

申请日：2018-11-01

申请人： University Health Network ,  Sinai Health System

发明人： DE CARVALHO, Daniel, Diniz ,  BRATMAN, Scott, Victor ,  CHAKRAVARTHY, Ankur, Ravinarayana ,  SINGHANIA, Rajat ,  BURGENER, Justin, Matthew ,  SHEN, Shu Yi

IPC分类号： C12Q1/6809 ,  C12M1/34 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N1/40 ,  G16B30/00

公开(公告)号：EP4435118A2

公开(公告)日：2024-09-25

申请号：EP24177800.0

申请日：2020-11-06

申请人： University Health Network ,  Van Andel Research Institute

发明人： WILSON, Samantha L ,  SHEN, Shu Yi ,  DINIZ DE CARVALHO, Daniel ,  HOFFMAN, Michael M. ,  TRICHE, Timothy J.

IPC分类号： C12Q1/6869

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12N15/1003

摘要： There is described herein, a method of capturing and analyzing cell-free methylated DNA in a sample. The method involves subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA. A predetermined amount of control synthetic DNA fragments are added to the sample. The control synthetic DNA fragments each have a known nucleic acid sequence that does not align to a target genome sequence, and at least some of the control synthetic DNA fragments are methylated. The sample is denatured, and cell-free methylated DNA and the control synthetic DNA fragments are captured using a binder selective for methylated polynucleotides. The captured DNA is amplified and sequenced.

公开(公告)号：EP4055183A1

公开(公告)日：2022-09-14

申请号：EP20884272.4

申请日：2020-11-06

申请人： University Health Network ,  Van Andel Research Institute

发明人： WILSON, Samantha L. ,  SHEN, Shu Yi ,  DINIZ DE CARVALHO, Daniel ,  HOFFMAN, Michael M. ,  TRICHE, Timothy J.

IPC分类号： C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6869