公开(公告)号：EP4425178A2

公开(公告)日：2024-09-04

申请号：EP24176389.5

申请日：2017-05-03

申请人： University Health Network ,  Sinai Health System

发明人： DINIZ DE CARVALHO, Daniel ,  SHEN, Shu Yi ,  SINGHANIA, Rajat

IPC分类号： G01N33/53

CPC分类号： C12N15/10 ,  C12N15/1093 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6886 ,  G01N33/5308 ,  C12Q2600/15420130101 ,  C12N15/1003

摘要： There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 ng of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.

公开(公告)号：EP4168574A1

公开(公告)日：2023-04-26

申请号：EP21825516.4

申请日：2021-06-18

申请人： University Health Network

发明人： BRATMAN, Scott Victor ,  BURGENER, Justin Matthew ,  DINIZ DE CARVALHO, Daniel

IPC分类号： C12Q1/6809 ,  C12Q1/6886 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/68

公开(公告)号：EP4435118A2

公开(公告)日：2024-09-25

申请号：EP24177800.0

申请日：2020-11-06

申请人： University Health Network ,  Van Andel Research Institute

发明人： WILSON, Samantha L ,  SHEN, Shu Yi ,  DINIZ DE CARVALHO, Daniel ,  HOFFMAN, Michael M. ,  TRICHE, Timothy J.

IPC分类号： C12Q1/6869

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12N15/1003

摘要： There is described herein, a method of capturing and analyzing cell-free methylated DNA in a sample. The method involves subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA. A predetermined amount of control synthetic DNA fragments are added to the sample. The control synthetic DNA fragments each have a known nucleic acid sequence that does not align to a target genome sequence, and at least some of the control synthetic DNA fragments are methylated. The sample is denatured, and cell-free methylated DNA and the control synthetic DNA fragments are captured using a binder selective for methylated polynucleotides. The captured DNA is amplified and sequenced.

公开(公告)号：EP4055183A1

公开(公告)日：2022-09-14

申请号：EP20884272.4

申请日：2020-11-06

申请人： University Health Network ,  Van Andel Research Institute

发明人： WILSON, Samantha L. ,  SHEN, Shu Yi ,  DINIZ DE CARVALHO, Daniel ,  HOFFMAN, Michael M. ,  TRICHE, Timothy J.

IPC分类号： C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6869

公开(公告)号：EP4412626A1

公开(公告)日：2024-08-14

申请号：EP22878051.6

申请日：2022-10-05

申请人： University Health Network

发明人： DINIZ DE CARVALHO, Daniel ,  LOO YAU, Helen ,  ETTAYEBI, Ilias

IPC分类号： A61K35/14 ,  A61K35/17 ,  A61P35/00 ,  C12N5/10 ,  C12N5/078 ,  C12N15/10 ,  C12N15/12 ,  C12N15/54 ,  C12N15/62 ,  C12N15/113

CPC分类号： A61K35/17 ,  A61P35/00 ,  C07K14/435 ,  C12N15/113 ,  C12N9/1029 ,  C12N9/16 ,  C07K14/4702 ,  C12N15/63 ,  C12Y304/19012 ,  C12Y203/01048 ,  C07K14/7051 ,  C07K2319/0320130101 ,  C12N15/1137 ,  C12N2310/2020170501 ,  C12N5/0636 ,  C12N2510/0020130101 ,  C12N2501/51520130101 ,  C12N2501/5320130101 ,  C12N2501/5120130101 ,  A61K45/06

公开(公告)号：EP4162070A1

公开(公告)日：2023-04-12

申请号：EP21817052.0

申请日：2021-06-03

申请人： University Health Network

发明人： DINIZ DE CARVALHO, Daniel ,  MEHDIPOUR, Parinaz ,  MARHON, Sajid

IPC分类号： C12Q1/6809 ,  A61K45/00 ,  A61P35/00

公开(公告)号：EP4133059A1

公开(公告)日：2023-02-15

申请号：EP21784330.9

申请日：2021-04-07

申请人： University Health Network

发明人： DINIZ DE CARVALHO, Daniel ,  HE, Housheng ,  ETTAYEBI, Ilias ,  SOARES, Fraser ,  LOO YAU, Helen

IPC分类号： C12N5/10 ,  A61K31/7088 ,  A61K31/7105 ,  A61K35/17 ,  A61K38/46 ,  A61P35/00 ,  A61P37/02 ,  C07K19/00 ,  C12N15/113 ,  C12N15/54 ,  C12N15/62 ,  C12N15/63 ,  C12N5/0783 ,  C12N9/22