公开(公告)号：EP1999256B1

公开(公告)日：2012-03-07

申请号：EP07758102.3

申请日：2007-03-07

申请人： Verenium Corporation

发明人： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC分类号： C12N9/10

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.

公开(公告)号：EP2432797A2

公开(公告)日：2012-03-28

申请号：EP10778424.1

申请日：2010-05-20

申请人： Verenium Corporation

发明人： WEINER, David, P. ,  SOLBAK, Arne, I., Jr. ,  MCCANN, Ryan

IPC分类号： C07H21/04

CPC分类号： C12N9/16 ,  Y02E50/17 ,  Y10T428/2982

摘要： This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

摘要翻译： 本发明涉及肌醇六磷酸酶，编码它们的多核苷酸，使用该多核苷酸和本发明的多肽，以及寻求多核苷酸和多肽的生产和分离的。 特别地，本发明提供具有多肽植酸酶活性高的温度条件下，和肌醇六磷酸酶没有暴露于高温后保持活性。 本发明提供这些都增加了胃的不稳定性进一步植酸酶。 本发明的肌醇六磷酸酶可以在食品中使用，以提高富植酸盐成分的供给值。 本发明的肌醇六磷酸酶可被配制为食品或饲料或补充剂要么到，E. G.，有助于植酸盐的消化。 本发明的食品或饲料可以是丸剂，液体，粉末等的形式。 在一个方面，本发明的肌醇六磷酸酶是反对造粒过程中的热变性稳定; 这降低了肌醇六磷酸酶的产物，而在体内效力和在进料活性的检测维护成本。

公开(公告)号：EP1999256A2

公开(公告)日：2008-12-10

申请号：EP07758102.3

申请日：2007-03-07

申请人： Verenium Corporation

发明人： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC分类号： C12N9/10

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.