公开(公告)号：EP1370674B1

公开(公告)日：2011-05-18

申请号：EP02706401.3

申请日：2002-02-21

申请人： Verenium Corporation

发明人： CALLEN, Walter ,  RICHARDSON, Toby ,  FREY, Gerhard ,  MILLER, Carl ,  KAZAOKA, Martin ,  MATHUR, Eric, J. ,  SHORT, Jay, M.

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/24 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2417 ,  A21D8/042 ,  C12N9/2414 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, and alkaline pH and increased temperature.

公开(公告)号：EP2074136A2

公开(公告)日：2009-07-01

申请号：EP08728602.7

申请日：2008-01-30

申请人： Verenium Corporation ,  Syngenta Participations AG

发明人： STEGE, Justin, T. ,  ABOUSHADI, Nahla ,  DJORDJEVIC, Gordana ,  BURKE, Ellen ,  LUGINBUHL, Peter ,  DYCAICO, Mark ,  RICHARDSON, Toby ,  POLAND, John ,  HEFNER, Ying ,  MILES, Stacy, Marie ,  CHANG, Cathy

IPC分类号： C07K5/00 ,  C12N9/00 ,  A61K48/00

CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12N9/2445 ,  C12P7/04 ,  C12P19/02 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta- glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β- xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts; for example, the invention provides a multi-enzyme system that can hydrolyze polysaccharides in a bagasse component of sugarcane processed in sugar mills. The invention provides enzymes for the bioconversion of lignocellulosic residues into fermentable sugars; and these sugars can be used as a chemical feedstock for the production of ethanol and fuels, including biofuels such as bioethanol, biopropanol, biobutanol and biodiesels.

公开(公告)号：EP2029739A2

公开(公告)日：2009-03-04

申请号：EP07873501.6

申请日：2007-05-29

申请人： Verenium Corporation

发明人： WEINER, David ,  VARVAK, Alexander ,  RICHARDSON, Toby ,  PODAR, Mircea ,  BURKE, Ellen ,  HEALEY, Shaun

IPC分类号： C12N9/88

CPC分类号： C12N9/88

摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

公开(公告)号：EP1999256A2

公开(公告)日：2008-12-10

申请号：EP07758102.3

申请日：2007-03-07

申请人： Verenium Corporation

发明人： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC分类号： C12N9/10

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.

公开(公告)号：EP1869174A2

公开(公告)日：2007-12-26

申请号：EP06748348.7

申请日：2006-03-10

申请人： Verenium Corporation

发明人： WEINER, David ,  VARVAK, Alexander ,  RICHARDSON, Toby ,  PODAR, Mircea ,  BURKE, Ellen ,  HEALEY, Shaun

IPC分类号： C12N9/88

CPC分类号： C12N9/88 ,  C12P7/42 ,  C12P13/222 ,  C12P13/225 ,  C12P13/24 ,  C12Y403/01005 ,  C12Y403/01023 ,  D06M16/003

摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X-NO2, Cl, Br, F, NH2, OH, H, alkyl at one or several o, m, and p positions R-H or alkyl

公开(公告)号：EP1999256B1

公开(公告)日：2012-03-07

申请号：EP07758102.3

申请日：2007-03-07

申请人： Verenium Corporation

发明人： BURKE, Ellen ,  HICKS, Paula, M. ,  LUGINBUHL, Peter ,  RICHARDSON, Toby ,  WEINER, David, P. ,  ZHAO, Lishan

IPC分类号： C12N9/10

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.

公开(公告)号：EP1928560A2

公开(公告)日：2008-06-11

申请号：EP06851338.1

申请日：2006-04-06

申请人： Verenium Corporation ,  Agentase, LLC ,  Life Science Research Israel Ltd.

发明人： ROBERTSON, Dan, E. ,  RICHARDSON, Toby ,  KUSTEDJO, Karen ,  AMITAI, Gabriel ,  LEJEUNE, Keith ,  BERBERICH, Jason ,  CHAPLIN, Jennifer, Ann ,  SINCLAIR, Jessica

IPC分类号： A62D3/02 ,  A62D101/04

CPC分类号： A62D3/02 ,  A62D2101/02 ,  C12N9/00 ,  C12N9/16

摘要： The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad-spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.